Transgenic Mouse Embryos
Transgenic Mouse Embryos - Related Videos
04:29
Ex Vivo Culture and Imaging of Oculomotor Slices from Transgenic Mouse Embryos
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2025
This video demonstrates the ex vivo culture and imaging of oculomotor slices from transgenic mouse embryos. The embryos are isolated and embedded in agarose. Using a vibratome, slices containing the oculomotor nuclei and eyes are obtained. The slices are maintained in culture and imaged using a fluorescence microscope.
17:39
Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes
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Cited by 56 •
2007
In this video, Nijole Jasinskiene demonstrates the methodology employed to generate transgenic Aedes aegypti mosquitoes, which are vectors for dengue fever. The techniques for correctly preparing microinjection needles, dessicating embryos, and performing microinjection are demonstrated.
02:52
Prenatal Mouse Embryos Retrieval: A Procedure to Harvest Embryos from Pregnant Mouse
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2025
This video demonstrates the retrieval of embryos from a pregnant mouse. The recovered perinatal embryos can be used as model systems for studying mammalian development.
03:07
Bioluminescence Imaging of Neuroinflammation in a Transgenic Mouse
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2025
Source: Breid, S., et. al. Bioluminescence Imaging of Neuroinflammation in Transgenic Mice After Peripheral Inoculation of Alpha-Synuclein Fibrils. J. Vis. Exp. (2017)This video demonstrates the bioluminescence imaging of neuroinflammation in transgenic mice, showcasing luciferase-luciferin reactions in activated astrocytes. The protocol enables non-invasive quantification of astrocytic activation, aiding studies on CNS inflammation and therapeutic efficacy.
09:10
Generation of Transgenic Hydra by Embryo Microinjection
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Cited by 34 •
2014
Stably transgenic Hydra are made by microinjection of plasmid DNA into embryos followed by random genomic integration and asexual propagation to establish a uniform line. Transgenic Hydra are used to track cell movements, overexpress genes, study promoter function, or knock down gene expression using RNAi.
FAQs
The introduced sequence is placed in a fertilized egg or incorporated into embryonic cells before development progresses. If the sequence becomes part of the genome, descendant cells may carry it as tissues form. This creates a way to follow how a genetic change is associated with outcomes across developing neural cells and, potentially, other tissues.
Targeted expression can increase the presence of a selected gene product, whereas disruption can reduce or eliminate its function in relevant neural cells. Comparing these altered conditions with an appropriate unaltered state helps connect gene activity to brain development, neuronal function, behavior, or neurological disease-related outcomes.
The developmental location and timing of the alteration influence which cells receive the introduced sequence as the embryo develops. Changes present in cells that contribute to developing tissues may shape later neural outcomes, while sequences incorporated early enough may also be inherited by developing tissues. These distinctions affect how researchers interpret tissue-level and organism-level results.
They allow a genetic manipulation to be examined across several levels of organization. Researchers can relate altered gene regulation to changes in neural cells, then assess how those cellular changes correspond to brain development, neuronal function, behavior, or disorder-related features. This progression helps connect molecular mechanisms with observable biological outcomes rather than treating them separately.
A typical workflow begins by selecting an introduced genetic sequence and delivering it into a fertilized egg or modifying embryonic cells. Researchers then examine whether the sequence becomes incorporated into the genome and follow its presence or activity during development. Subsequent analyses can relate the alteration to neural, cellular, behavioral, or disease-relevant outcomes.
They are useful when investigators need a controlled developmental model for testing how a gene influences the nervous system. Applications include examining brain development, neuronal function, behavior, neurological disorders, and gene regulation. The same approach can also support evaluation of experimental therapies by showing how molecular changes relate to cellular or organism-level effects.
Studies may show whether an introduced sequence is incorporated, whether it changes gene expression or function, and how that alteration relates to developing tissues. In neuroscience, the resulting evidence can connect molecular events with neural-cell behavior, brain development, behavior, or disease-associated features. Interpretation depends on considering outcomes at molecular, cellular, and organism levels together.