Sensory Neuron Recording
Sensory Neuron Recording - Related Videos
03:34
Establishing a Whole-Cell Patch Clamp for Electrophysiological Recording from Vomeronasal Sensory Neurons
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2025
The protocol video describes establishing a whole-cell patch clamp for electrophysiological recording from the sensory neurons of the vomeronasal organ (VNO) in mice. A coronal VNO slice with fluorescently labeled sensory neurons is placed in a recording setup. A labeled neuron is patched with a recording pipette in a whole-cell configuration for electrophysiological recording in response to chemical stimuli.
06:22
Perforated Patch-Clamp Recording of Olfactory Sensory Neurons in Mice
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2025
This video demonstrates electrophysiological recording of olfactory sensory neurons (OSNs) in mice using a perforated patch-clamp technique. The olfactory epithelium, containing OSNs with fluorophore-tagged odor receptors, is isolated from a mouse and secured in a recording chamber. A recording pipette is clamped to the dendritic knob of an OSN, and nystatin within the pipette perforates the membrane to form electrical connectivity with the cell. Finally, another pipette is used to deliver an...
02:50
Perforated Patch-Clamp Recordings of Inner Ear Sensory Neuron Cell Bodies
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2025
This video demonstrates the patch-clamp technique for measuring ionic currents in inner ear sensory neuron cell bodies. It details the preparation and use of a polished patch-clamp pipette filled with an internal solution containing amphotericin B, which perforates the membrane to allow ion movement while preserving intracellular conditions. Voltage pulses are applied to trigger and record ion channel activity, providing insights into their properties and behavior.
09:40
Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents
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Cited by 5 •
2013
We describe the dissection of the nervous system of the marine sea hare Aplysia after anesthesia, the isolation of neurons for short term-tissue culture, and recordings of single cell ion currents via the patch clamp technique.
11:53
Intracellular Recording, Sensory Field Mapping, and Culturing Identified Neurons in the Leech, Hirudo medicinalis
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Cited by 14 •
2013
This article describes three nervous system preparations using leeches: intracellular recording from neurons in ventral ganglia, culturing neurons from ventral ganglia, and recording from a patch of innervated skin to map sensory fields.
FAQs
Stimulus intensity, timing, and modality can be related to distinct patterns of sensory neuron activity. During a recording, researchers examine corresponding changes in membrane potential or action potential firing as controlled physical or chemical stimuli activate receptors. This comparison shows how sensory input is translated into neural signals rather than treated as a single undifferentiated response.
The two measurements provide complementary views of signaling. Changes in membrane potential show how the neuron's electrical state responds to receptor activation, whereas action potential firing records the resulting neural events. Monitoring either or both helps investigators connect an applied stimulus with the electrical behavior that carries sensory information toward the central nervous system.
Controlled stimulation allows neural responses to be compared with known changes in the input. By linking stimulus timing, intensity, or modality to recorded electrical activity, researchers can determine how sensory information is encoded. This design is especially useful when comparing responses associated with touch, pain, temperature, or other sensory modalities.
A basic experiment places an electrode near or within a sensory neuron, applies a selected physical or chemical stimulus, and records the resulting electrical changes. The recording may track membrane potential, action potential firing, or both. Keeping the stimulus controlled makes it possible to relate the measured response to the sensory condition being tested.
These recordings expose how sensory signals are transformed before they reach the central nervous system. Researchers can examine whether a physical or chemical stimulus is associated with particular changes in membrane potential or firing, then relate those changes to stimulus intensity, timing, and modality. The result is a physiological link between receptor activation and transmitted sensory information.
In neuroscience, the method supports investigations of touch, pain, temperature, and other sensory systems. Findings can clarify how neurons encode sensory inputs and provide context for studying sensory disorders. Because the measurements connect controlled stimulation with neuronal activity, they can also inform research on neural processes relevant to therapeutic targets.