Spinal Cord Gene Expression
Spinal Cord Gene Expression - Related Videos
05:12
Subpial Gene Delivery in the Mouse Spinal Cord to Evaluate Gene Expression
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2025
Source: Tadokoro, T., et al. Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice. J. Vis. Exp. (2017)This video demonstrates a subpial injection technique for gene delivery to the spinal cord in mice. The procedure involves exposing the dura mater, puncturing the pia mater, and injecting a recombinant adeno-associated virus (rAAV) carrying a fluorescent protein-expressing gene into the subpial space. Following injection and recovery, the virus facilitates gene expression in...
04:59
Spinal Cord Electrophysiology
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Cited by 5 •
2010
A demonstration of the isolation of neonatal mouse spinal cord for electrophysiologic studies.
08:57
Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats
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Cited by 55 •
2012
We describe two tactile sensory testing methods for acute or chronic periods of spinal cord injury in rats. These validated procedures can detect the development and maintenance of allodynia-like sensations.
08:39
Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord
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Cited by 18 •
2013
Viral vectors allow for targeted gene manipulation. We demonstrate a method for conditional gene expression or ablation in the mouse spinal cord, using stereotaxic injection of a viral vector into the dorsal horn, a prominent site of synaptic contact between primary somatosensory afferents and neurons of the central nervous system.
08:41
Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord
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Cited by 21 •
2018
Intraspinal injection of recombinase dependent recombinant adeno-associated virus (rAAV) can be used to manipulate any genetically labelled cell type in the spinal cord. Here we describe how to transduce neurons in the dorsal horn of the lumbar spinal cord. This technique enables functional interrogation of the manipulated neuron subtype.
FAQs
Transcription factors bind regulatory DNA sequences and help determine which genes RNA polymerase copies into messenger RNA. Their activity can selectively promote or suppress transcription, allowing spinal cord cells to adjust production of proteins and signaling molecules. This regulatory control links cellular signals to gene activity and helps explain changing neural states during development or after injury.
Regulatory DNA sequences provide control regions that influence whether particular genes are accessible for transcription. RNA polymerase then copies selected genetic information into messenger RNA, creating an intermediate that supports later protein production. Together, these components connect gene-level control with the molecular outputs required for neural development, signaling, function, and repair.
Neurons, glial cells, and other spinal cord cell types can activate different sets of genes because their regulatory programs and cellular roles differ. As a result, each population may produce distinct proteins and signaling molecules. Examining these cell-specific patterns helps distinguish processes supporting neural communication from those involved in cellular support, development, or responses to damage.
Expression patterns can shift as the spinal cord develops, receives changing cellular signals, or responds to injury. These influences alter which genes are activated or suppressed at a given time. Comparing patterns across these conditions can reveal how molecular programs support normal development and function, and how they change during responses associated with spinal cord damage or disease.
Molecular profiles can show which genes and signaling programs differ across spinal cord conditions, cell types, or states. Such differences may point to disease mechanisms by linking altered expression with disrupted neural function or injury responses. The same profiles can also help identify biomarkers and molecular targets for strategies intended to support repair or treatment development.
Gene expression patterns provide molecular context for spinal cord functions involved in motor control and sensory processing. Researchers can relate regulated gene activity to the cells and signaling molecules that support these functions, then examine how those patterns change during development, injury, or disease. This connection helps translate cellular regulation into explanations of broader nervous-system behavior.