Spinal Cord Injection
Spinal Cord Injection - Related Videos
02:25
A Viral Vector Injection into the Rat Spinal Cord for Retrograde Neuronal Tracing
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2025
Source: Keefe, K. M., et al. Direct Injection of a Lentiviral Vector Highlights Multiple Motor Pathways in the Rat Spinal Cord. J. Vis. Exp. (2019).This video demonstrates the injection of a highly efficient retrograde transport (HiRet) viral vector into the rat spinal cord to trace neuronal pathways. The virus has glycoproteins for synaptic entry and a fluorescent protein coding gene. The needle is inserted into the spinal cord, and the virus suspension is administered at a controlled rate.
08:57
Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats
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Cited by 55 •
2012
We describe two tactile sensory testing methods for acute or chronic periods of spinal cord injury in rats. These validated procedures can detect the development and maintenance of allodynia-like sensations.
08:57
Experimental Demyelination and Remyelination of Murine Spinal Cord by Focal Injection of Lysolecithin
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Cited by 79 •
2015
Demyelinating diseases can be modeled in animals by focal application of lysolecithin into the CNS. A single injection of lysolecithin into mouse spinal cord produces a lesion that spontaneously repairs over time. The goal is to study factors involved in de- and remyelination, and to test agents for enhancing repair.
08:39
Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord
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Cited by 18 •
2013
Viral vectors allow for targeted gene manipulation. We demonstrate a method for conditional gene expression or ablation in the mouse spinal cord, using stereotaxic injection of a viral vector into the dorsal horn, a prominent site of synaptic contact between primary somatosensory afferents and neurons of the central nervous system.
04:18
Injecting Self-Assembling Peptides and Neural Precursor Cells for Spinal Cord Repair in a Rat Model
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2025
Source: Zweckberger, K., et al. Synergetic Use of Neural Precursor Cells and Self-assembling Peptides in Experimental Cervical Spinal Cord Injury. J. Vis. Exp. (2015).This protocol demonstrates a targeted injection approach for spinal cord injury repair in a rat model. It involves delivering self-assembling peptides (SAPs) into the lesion core to create a supportive scaffold, followed by neural precursor cell (NPC) injections around the lesion to remodel the tissue.
FAQs
The route determines where the administered material first acts. Intrathecal delivery places it in cerebrospinal fluid, whereas direct tissue injection deposits it in a defined neural region. This distinction lets investigators compare effects mediated through the fluid environment with effects concentrated in spinal cord tissue, making route selection central to interpreting neural responses.
Injection location, volume, and delivery rate are core experimental variables because they determine how consistently the material reaches the intended site and how safely tissue is exposed. Holding these factors constant improves reproducibility between experiments, while documenting changes helps distinguish a therapy’s biological effect from differences in administration.
Local spinal delivery can concentrate an agent near the central nervous system while limiting exposure to other tissues. That balance is important when researchers want to examine a regional neural effect or evaluate an experimental therapy without treating the entire body as the primary exposure site. The chosen route determines how local that action can be.
Planning starts with choosing the intended route, neural location, and material to be delivered. The investigator then selects an appropriate needle or catheter approach and specifies the injection volume and delivery rate. These parameters should be controlled and recorded carefully, because consistent administration supports tissue safety and makes results easier to reproduce and interpret.
Spinal cord injection supports studies of neural signaling, pain, spinal cord injury, and neurodegenerative disease. By delivering an agent through cerebrospinal fluid or into a defined neural region, researchers can examine how localized exposure affects the central nervous system. The technique also provides a way to evaluate experimental interventions in disease- or injury-related contexts.
Researchers can use spinal cord injection to assess experimental gene therapies and regenerative approaches by placing biological agents near or within relevant spinal neural regions. The resulting studies can examine whether a localized intervention produces effects in the central nervous system. Careful control of route, location, volume, and rate is essential when comparing treatment outcomes.