Vascular Lesion Formation
Vascular Lesion Formation - Related Videos
05:51
A Human Ex Vivo Atherosclerotic Plaque Model to Study Lesion Biology
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Cited by 13 •
2014
Atherosclerosis is a chronic inflammatory process. This manuscript illustrates an easy to use ex vivo model to investigate fresh carotid or coronary artery plaques. The ex vivo model allows for the investigation of potential substances on the inflammatory milieu in human atherosclerotic lesions and results can be analyzed by various methods.
06:43
Quantitative Analysis and Characterization of Atherosclerotic Lesions in the Murine Aortic Sinus
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Cited by 51 •
2013
We describe procedures to quantify and characterize atherosclerotic lesions in mouse models using precision sectioning of the aortic sinus and ascending aorta combined with histochemical and immunohistochemical analysis.
08:16
Comparative Lesions Analysis Through a Targeted Sequencing Approach
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2019
This article describes a method to identify clonal and subclonal alterations among different specimens from a given patient. Although the experiments described here focus on a specific tumor type, the approach is broadly applicable to other solid tumors.
11:18
Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice
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Cited by 29 •
2013
Here we report an experimental technique of fluorescence intravital microscopy to visualize heterotypic platelet-neutrophil interactions on the activated endothelium during vascular inflammation and thrombus formation in live mice. This microscopic technology will be valuable to study the molecular mechanism of vascular disease and to test pharmacologic agents under pathophysiological conditions.
11:45
Generation and 3-Dimensional Quantitation of Arterial Lesions in Mice Using Optical Projection Tomography
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Cited by 5 •
2015
Ex vivo analysis of arterial lesions from animal models of cardiovascular disease classically relies on histological and immunohistochemical techniques. These provide 2-dimensional measurements in 3-dimensional lesions. This manuscript describes the generation of arterial lesions for quantitative analysis in 3-dimensions using optical projection tomography.
FAQs
Endothelial injury can disturb the vessel lining and initiate changes in vessel structure and function. In the surrounding tissue, these changes may promote remodeling, in which vessels alter their organization or architecture, and can weaken blood-brain barrier integrity. Studying this sequence helps researchers connect an initial vascular disturbance with later leakage, thrombosis, or hemorrhage.
Altered blood flow and inflammation act as interacting drivers of vascular change. Disturbed flow can influence how vessels remodel, while inflammation may modify both vessel structure and the surrounding neural tissue. Together, these processes help explain why lesion development is not limited to the vessel wall and may ultimately affect neural function.
Blood-brain barrier integrity is important because it regulates whether substances remain contained within cerebral vessels or reach surrounding tissue. When this barrier weakens during lesion formation, leakage can occur and potentially contribute to tissue disruption. Measuring barrier changes therefore provides a way to connect vascular abnormalities with neurological consequences and lesion progression.
Experimental models allow researchers to examine lesion development under controlled conditions and to test how cellular or molecular drivers influence the process. Investigators can follow changes in vessel structure, barrier integrity, and surrounding tissue over time. These models are particularly useful for comparing mechanisms and evaluating potential strategies for prevention or treatment.
Imaging methods can track lesion growth and characterize changes in affected vessels and surrounding tissue. Repeated or complementary imaging observations may help researchers assess whether a lesion is remodeling, leaking, or associated with hemorrhage. Such information supports investigation of disease progression and provides measurable outcomes for evaluating diagnostic or therapeutic approaches.
In neuroscience, this research provides context for aneurysms, arteriovenous malformations, and cerebral small-vessel disease. Examining shared processes such as endothelial injury, altered flow, inflammation, and barrier disruption helps investigators relate vascular abnormalities to neural dysfunction. The same framework also supports efforts to identify molecular drivers and develop approaches for diagnosis, prevention, and treatment.