Work Done

Work done is the energy transferred when a force causes an object to move through a displacement, making it a central concept in physics and mechanics. For a constant force, work is calculated as W = Fd cos θ, where F is the force magnitude, d is the displacement, and θ is the angle between them; only the force component parallel to the motion transfers energy. Positive work increases an object’s kinetic or potential energy, while negative work removes energy, as with friction. Understanding work done supports analysis of machines, motion, power, and energy conservation in systems ranging from laboratory experiments to engineering applications.

Work Done - Related Videos

Research

JoVE Journal - Immunology and Infection

Measurement of Tactile Allodynia in a Murine Model of Bacterial Prostatitis

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Cited by 23 •

2013

Infection of the prostate may be a contributing factor in mediating pelvic pain in chronic prostatitis. We describe the procedure for preparation of standardized bacterial inoculum, instillation of bacteria into the urethra of male mice and methodology for measuring tactile allodynia in mice over time.

Research

JoVE Journal - Biology
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Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy

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Cited by 5 •

2021

Here we show how to use the sandwich freezing device for rapid freezing of biological specimens, including bacteria, yeast, cultured cells, isolated cells, animal and human tissues, and viruses. We also show how to prepare specimens for ultrathin sectioning after rapid freezing.

A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications

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Cited by 3 •

2022

The liquid biopsy has revolutionized our approach to oncology translational studies, with sample collection, quality, and storage being crucial steps for its successful clinical application. Here we describe a standardized and validated protocol for downstream circulating-free DNA applications that can be applied in most translational research laboratories.

Research

JoVE Journal - Engineering
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A System to Create Stable Nanoparticle Aerosols from Nanopowders

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Cited by 4 •

2016

We designed and developed an effective nanopowder aerosolization setup and operating protocol. The system generated nanoparticle aerosols with stable number concentrations and size distributions for long durations, requiring only small quantities of test material (min. 200 mg).

Mechanical Dissociation of Tissues for Single Cell Analysis Using a Motorized Device

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Cited by 3 •

2023

A general protocol for the combined enzymatic and semi-automated mechanical dissociation of tissues to generate single-cell suspensions for downstream analyses, such as flow cytometry, is provided. Instructions for the fabrication, assembly, and operation of the low-cost mechanical device developed for this protocol are included.

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