The authors have nothing to disclose.
1M Tris-HCl –pH 7.5 | Thermo Scientific | 15567-027 | |
1M Tris-HCl –pH 8 | Thermo Scientific | 15568-025 | |
0.5M EDTA | Thermo Scientific | AM9260G | |
5M NaCl | Sigma | 1001385276 | |
Triton X-100 | Sigma | 1001412354 | |
Sodium-Deoxycholate | Sigma | 1001437582 | |
SDS | Thermo Scientific | 15525-017 | |
Sodium-Bicarbonate | Fisher Scientific | S233-500 | |
100% EtOH | NA | NA | |
V-bottom 96 well plate (AB 1400) | Thermo Scientific | AB-1400-L | |
Gilson P2 pipetman | Mandel | F144801 | |
Gilson P10 pipetman | Mandel | F144802 | |
Gilson P20 pipetman | Mandel | F123600 | |
Gilson P100 pipetman | Mandel | F123615 | |
Gilson P200 pipetman | Mandel | F123601 | |
Gilson P1000 pipetman | Mandel | F123603 | |
Micrococcal Nuclease | NEB | M0247S | |
Thermo Mixer C (Heating block mixer) | Eppendorf | 5382000023 | |
Multi 12-channel Pippet P20 | Rainin | 17013803 | |
Multi 12-channel Pippet P200 | Rainin | 17013805 | |
1.5 ml LoBind tube | Eppendorf | 22431021 | |
0.5 ml LoBind tube | Eppendorf | 22431005 | |
Plastic Plate Cover | Edge Bio | 48461 | |
PCR cover | Bio Rad | MSD-1001 | |
Aluminum Plate Cover | Bio Rad | MSF-1001 | |
Elution buffer (EB buffer) | Qiagen | 19086 | |
1M DTT | Sigma | 646563 | |
Eppendorf 5810R centrifuge | Eppendorf | 22625004 | |
Ultrapure water | Thermo Scientific | 10977-015 | |
Protein G dynabeads | Thermo Scientific | 10001D | |
Protein A dynabeads | Thermo Scientific | 10001D | |
Protease inhibitor Cocktail | Calbiochem | 539134 | |
Rotating platform | Mandel | 1b109-12052010 | |
Plate magnet | Alpaqua | 2523 | |
Domed 12-cap strip | Bio Rad | TCS1201 | |
Buffer G2 | Qiagen | 1014636 | |
Protease | Qiagen | 19155 | |
Tube Magnet (DynaMag-2) | Thermo Scientific | 12321D | |
Tube Magnet (DynaMag-2) | LabCore | 730-006 | |
V shape Plastic reservoir | Mandel | S0100A | |
Vortex | Mandel | C1801 | |
Mini Fuge | Mettler Toledo | XS2035 | |
Analytical scale | Mandel | LB109-12052010 | |
Quick Ligation Reaction Buffer, 5X | NEB | B6058S | |
DNA Polymerase I, Large (Klenow) Fragment | NEB | M0210S | |
Klenow Fragment (3'-5' exo) | NEB | M0212S | |
T4 DNA Polymerase | NEB | M0203S | |
T4 Polynucleotide Kinase | NEB | M0201S | |
Deoxynucleotide Solution mix, 10mM | NEB | N0447S | |
dATP Solution 10mM | NEB | N0440S | |
Quick T4 DNA Ligase | NEB | M0202T | |
Adenosine 5'-Triphosphate (ATP), 25mM | NEB | P0756S | |
DNA Polymerase | Thermo Scientific | F549L | |
NEBuffer 2 | NEB | B7002S | |
Hank's buffered salt solution | Thermo Scientific | 14025076 | |
Fetal bovine serum | Thermo Scientific | A3160702 | |
phosphate buffer saline | Thermo Scientific | 10010023 | |
H3K4me3 | Cell Signaling | 9751S | |
H3K4me1 | Diagenode | C15410037 | |
H3K27me3 | Diagenode | pAb-069-050 | |
H3K36me3 | Abcam | ab9050 | |
H3K9me3 | Diagenode | C15410056 | |
SeraMag super-paramagnetic beads |