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Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis
JoVE Journal
Genetics
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JoVE Journal Genetics
Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis

Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis

DOI:
10.3791/56085-v

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10:05 min

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December 12, 2017

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, , , ,
1Department of Microbiology and Immunology, Michael Smith Laboratories Centre for High-Throughput Biology,University of British Columbia, 2Canada’s Michael Smith Genome Science Center,BC Cancer Agency

Chapters

  • 00:05Title
  • 00:51Cell Lysis and Chromatin Digestion for Nucleosome Density ChIP-se
  • 03:21Immunprecipitation Reaction for Nucleosome Density ChIP-seq
  • 04:33Washes and Elution for Nucleosome Density ChIP-seq
  • 07:31Results: Evaluation of Nucleosome Density ChIP-seq Libraries
  • 08:50Conclusion

Summary

Automatic Translation

Automatic Translation

We present a modified native chromatin immunoprecipitation sequencing (ChIP-seq) methodology for the generation of sequence datasets suitable for a nucleosome density ChIP-seq analytical framework integrating micrococcal nuclease (MNase) accessibility with histone modification measurements.

Tags

Keywords: Native Chromatin ImmunoprecipitationNucleosome Density AnalysisEpigeneticsEpigenomic FeaturesChromatin SignaturesMNaseCell PreparationLysis BufferMNase DigestionEDTA

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