Patient-specific Cardiomyocytes
Patient-specific Cardiomyocytes - Related Videos
13:42
Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes
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Cited by 3 •
2013
The presented method offers a way to detect functional effective cardiotropic autoantibodies in the plasma of patients with dilated cardiomyopathy, irrespective of the specific antigen, by analysing the impact of isolated patient immunoglobulin on cellular shortening and intracellular calcium transients in isolated rat cardiomyocytes.
12:09
Patient-specific Modeling of the Heart: Estimation of Ventricular Fiber Orientations
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Cited by 14 •
2013
A methodology to estimate ventricular fiber orientations from in vivo images of patient heart geometries for personalized modeling is described. Validation of the methodology performed using normal and failing canine hearts demonstrate that that there are no significant differences between estimated and acquired fiber orientations at a clinically observable level.
08:03
Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover
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Cited by 25 •
2016
Quantification of cardiomyocyte turnover is challenging. The protocol described here makes an important contribution to this challenge by enabling accurate and sensitive quantification of neo-cardiomyocyte nuclei generation and nuclei ploidy.
07:50
Isolation and Culture of Neonatal Mouse Cardiomyocytes
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Cited by 187 •
2013
Primary mouse cardiomyocyte cultures are one of the pivotal tools for the investigation of myofibrillar organization and function. The following protocol describes the isolation and culture of primary cardiomyocytes from neonatal mouse hearts. The resulting cardiomyocyte cultures may be subsequently used for a variety of biomechanical, biochemical and cell-biological assays.
12:10
Measuring Fast Calcium Fluxes in Cardiomyocytes
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Cited by 3 •
2011
We present a method to isolate rapid (microsecond) calcium events from slower fluxes in living cells using laser scanning confocal microscopy. The method measures fluorescence intensity fluctuations of calcium indicators by recording line scans of several hundred pixels in a cell. Histogram analysis allows us to isolate the time scales of different calcium fluxes.
FAQs
The transition from adult cells to cardiomyocytes depends on two linked stages: reprogramming produces induced pluripotent stem cells, and directed differentiation uses developmental signaling cues plus culture conditions to promote cardiac identity. This sequence matters because the starting cells are first reset to a pluripotent state and then guided toward a specific cardiac lineage.
Patient-associated genetic features remain valuable because they allow the cells to reflect disease-linked biology from the individual who supplied the starting material. In disease modeling, researchers can examine abnormal electrical or contractile activity and relate those findings to the underlying condition. This preserves genetic context that is central to biologically relevant cardiac investigation.
Electrical and contractile behavior provide complementary readouts of how a cardiac model functions. Abnormal activity can support disease investigation, while exposure to candidate drugs can help assess efficacy and cardiotoxicity. Using both functional dimensions connects cellular phenotype with pharmacological testing, making the cells useful for evaluating whether a treatment improves or disrupts cardiac performance.
A basic workflow starts with obtaining adult cells from the individual, reprogramming them into induced pluripotent stem cells, and applying developmental signaling cues under culture conditions that promote cardiac identity. The resulting cells can then serve as experimental material for studying function, disease-associated behavior, or drug responses. Each stage links the patient’s starting biology to the final model.
In bioengineering, these cells support tissue-engineered heart models, extending analysis beyond isolated cellular behavior. They also support precision medicine by preserving patient-associated features while researchers investigate cardiac function, disease mechanisms, and treatment responses. Their value lies in connecting an individual’s biology with engineered experimental systems for evaluating function and exploring therapeutic possibilities.
By linking patient-associated genetics with electrical, contractile, and drug-response observations, researchers can build a patient-centered evidence base for precision medicine. The resulting information can inform investigation of personalized therapies while revealing how a particular individual’s cardiac cells behave under disease or treatment conditions. This connects disease modeling, pharmacological testing, and engineered cardiac systems.