Bacterial Pellet
Bacterial Pellet - Related Videos
11:25
Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing
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Cited by 27 •
2014
A rapid bacterial pellet preparation from a positive blood culture can be used as a sample for applications such as identification by MALDI-TOF, Gram staining, antibiotic susceptibility testing and PCR-based test. The results can be rapidly communicated to clinicians to improve the outcome of patients suffering from bloodstream infections.
07:37
Sorting of Streptomyces Cell Pellets Using a Complex Object Parametric Analyzer and Sorter
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Cited by 12 •
2014
Liquid-grown Streptomyces cultures are characterized by mycelial pellets that are heterogeneous in size. We here describe a method to analyze and sort such pellets in a high-throughput manner. These pellets can be used for further analyses, which will provide leads to understand and control growth heterogeneity.
08:52
Method to Produce Durable Pellets at Lower Energy Consumption Using High Moisture Corn Stover and a Corn Starch Binder in a Flat Die Pellet Mill
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Cited by 50 •
2016
In this study, a protocol was developed to produce good quality pellets using a flat die pellet mill at reduced specific energy consumption testing high-moisture corn stover and a starch based binder. The results indicated that adding a corn starch binder improved the pellet durability, reduced percent fines and decreased specific energy consumption.
12:10
Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells
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Cited by 17 •
2017
Here, we propose a protocol for chondrogenic differentiation from cord blood mononuclear cell-derived human induced pluripotent stem cells.
10:12
Extraction of Organochlorine Pesticides from Plastic Pellets and Plastic Type Analysis
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Cited by 10 •
2017
Microplastics act as vector of potentially toxic organic contaminants with unpredictable effects. This protocol describes an alternative methodology for assessing the levels of organochlorine pesticides adsorbed on plastic pellets and identifying the polymer chemical structure. The focus is on pressurized fluid extraction and attenuated total reflectance Fourier transform infrared spectroscopy.
FAQs
During centrifugation, centrifugal force moves bacterial cells and other dense particles toward the bottom of the tube. As particles accumulate, they compact into the pellet, while the less concentrated surrounding liquid remains above as supernatant. This separation lets researchers handle the cellular material independently from the liquid phase.
Pellet size and appearance can provide an approximate indication of cell recovery and culture density, but they should not be treated as exact measurements. Differences in compactness or visible mass can reflect how much material was collected, making the pellet useful for initial assessment before resuspension or downstream analysis.
The supernatant contains the liquid surrounding the concentrated cells, so removing it isolates the material collected at the tube bottom. Researchers can then resuspend the remaining pellet in an appropriate liquid for a chosen downstream task. This separation prevents surrounding culture liquid from being carried unnecessarily into later analyses.
A basic workflow starts with a liquid bacterial culture and centrifugation in a tube. Once the cells have compacted, the supernatant is removed, leaving the pellet available for resuspension. The resuspended material can then be directed toward washing, microscopy, protein extraction, DNA or RNA isolation, or biochemical analysis.
Concentrating cells into a pellet gives researchers a manageable form of material for several biological workflows. After the supernatant is removed, the recovered cells can be resuspended and used for washing or microscopy, or processed for protein extraction, DNA or RNA isolation, and downstream biochemical analysis.
The recovered cellular material can support both observational and biochemical work. Resuspended cells may be examined by microscopy or subjected to washing, while extracted material can be used for protein studies, DNA or RNA isolation, or other biochemical analyses. Thus, the same collection step can precede different investigations depending on the research objective.