Erythroblast Enucleation
Erythroblast Enucleation - Related Videos
09:40
Identification of a Murine Erythroblast Subpopulation Enriched in Enucleating Events by Multi-spectral Imaging Flow Cytometry
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Cited by 6 •
2014
The present protocol describes a novel method of identifying a population of enucleating orthochromatic erythroblasts by multi-spectral imaging flow cytometry, providing a visualization of the erythroblast enucleation process.
04:19
Robotic Enucleation of Esophageal Leiomyoma
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2026
This manuscript describes the surgical technique for robotic enucleation of an esophageal leiomyoma, serving as a reference for similar cases.
05:30
Mouse Eye Enucleation for Remote High-throughput Phenotyping
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Cited by 19 •
2011
The dissection technique illustrates enucleation of the mouse eye for tissue fixation to perform phenotyping in high-throughput screens.
00:05
Robotic Enucleation of an Intra-Pancreatic Insulinoma in the Pancreatic Head
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Cited by 4 •
2020
Here, we present a robotic approach to enucleate an insulinoma in the pancreatic head.
05:29
A Highly Reproducible and Straightforward Method to Perform In Vivo Ocular Enucleation in the Mouse after Eye Opening
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Cited by 21 •
2014
The removal of eyes, also called enucleation, provides a useful strategy to study aspects of visual, cross-modal, and developmental plasticity along the mammalian visual system since it induces irreversible partial (monocular) or complete (binocular) vision loss. Here we describe a highly reproducible and straightforward approach to perform in vivo enucleation.
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The nucleus first condenses and shifts toward one side of the late-stage erythroblast. This polarized arrangement helps organize the cell for physical separation into two distinct products: the nascent reticulocyte and the extruded nuclear pyrenocyte. Nuclear repositioning therefore provides essential spatial organization before cytoskeletal remodeling completes the enucleation process.
An actomyosin-based contractile apparatus works with broader cytoskeletal remodeling to divide the late-stage cell. Its contraction helps separate the developing reticulocyte from the nuclear pyrenocyte after the nucleus has moved toward the cell periphery. This coordinated mechanical activity is central to completing nuclear extrusion rather than merely condensing the nucleus.
Removing the nucleus and other organelles produces a flexible, streamlined red blood cell. These structural changes support the specialized form of the mature erythrocyte and its role in oxygen transport. Enucleation is therefore not only a loss of nuclear material; it is part of the broader cellular remodeling that creates a hemoglobin-rich, functionally optimized blood cell.
A study of this process can distinguish the late-stage erythroblast, the nascent reticulocyte, and the extruded nuclear pyrenocyte. It can also follow nuclear condensation, movement to one side of the cell, cytoskeletal remodeling, and actomyosin-driven separation. These features connect visible cellular changes with the transition from developing erythroblast to reticulocyte.
Examining erythroblast enucleation helps clarify normal erythropoiesis, the developmental pathway that produces red blood cells. The process also provides a framework for investigating anemia and red blood cell disorders, where normal maturation may be relevant. Understanding this terminal developmental event can therefore connect cellular mechanisms with broader questions about blood-cell production and function.
Engineered blood production must account for the late developmental remodeling that produces a reticulocyte and ultimately a mature erythrocyte. Erythroblast enucleation offers a key biological context for evaluating whether generated cells undergo nuclear extrusion, organelle removal, and the structural streamlining associated with oxygen transport. Its study can therefore inform efforts to reproduce normal red blood cell development.