Water Aspirator

A water aspirator is a laboratory vacuum device that uses flowing water to generate suction, providing a simple way to remove air or liquids from a vessel. As water accelerates through a narrow opening, the resulting pressure decrease draws gas or fluid through connected tubing; vacuum strength depends on water flow and local water pressure. In biology laboratories, water aspirators support vacuum filtration, liquid aspiration, and degassing or drying steps during sample preparation, although they require attention to backflow and wastewater disposal. Their low cost and straightforward operation make them useful for routine procedures where a moderate, water-driven vacuum is sufficient.

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Fluid Aspiration from Mouse Glomeruli: A Method to Aspirate Glomerular Fluid from Mouse Using Two-Photon Mediated Micropuncture

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2023

This video showcases fluid aspiration from mouse glomeruli using the two-photon mediated micropuncture technique. The technique provides access to the living mouse’s glomeruli and thus helps gain insights into renal physiology.

Femoral Bone Marrow Aspiration in Live Mice

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Cited by 18 •

2014

This protocol describes a procedure for serial sampling of femoral bone marrow (BM) without requiring the sacrifice of mice. This procedure facilitates longitudinal studies of the BM composition of mice over time and provides serial access to cells within the BM for ex vivo and transplantation studies.

A Technique for Percutaneous Epididymal Spermatozoa Aspiration and Processing

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2026

Source:Li Xiaoming1, Liu Xiao1, Li Haiyang1, Cai Xiaohua1, Ou Jianping11Center for Reproductive Medicine, The Third Affiliated Hospital of Sun Yat-sen University.This video demonstrates the percutaneous aspiration of epididymal spermatozoa from a patient with azoospermia, a condition characterized by the absence of spermatozoa in the semen. The procedure involves disinfecting the scrotal area, aspirating epididymal fluid using a syringe with a specialized culture solution and processing the...

Fly Aspirator Tube: A Simple Tool to Transfer Live Drosophila

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2023

This video describes one method to build a fly aspirator, which can be used to transfer insects between enclosures without anesthesia. The aspirator in the example protocol was prepared for an experiment to quantify individual grooming...

Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness

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Cited by 32 •

2012

Here we describe a quick and simple method to measure cell stiffness. The general principle of this approach is to measure membrane deformation in response to well-defined negative pressure applied through a micropipette to the cell surface. This method provides a powerful tool to study biomechanical properties of substrate-attached cells.

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