Endothelial Cell Adhesion
Endothelial Cell Adhesion - Related Videos
10:24
Quantitation of Endothelial Cell Adhesiveness In Vitro
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Cited by 10 •
2015
We report an in vitro method that allows the quantitation of the actual number of adhesive cells within an endothelial cell monolayer.
03:10
Micro-Parallel Plate Flow Chamber Model for Studying Bacterial Adhesion to Human Endothelial Cells
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2026
Source: Claes, J., et al, In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions. J. Vis. Exp. (2015).This video demonstrates a method for studying host-pathogen interactions using a micro-parallel plate flow chamber seeded with human primary endothelial cells. Following endothelial cell activation by an ionophore and perfusion with fluorescently labeled bacteria, the cells release von Willebrand factor (VWF), which binds bacterial adhesins under shear...
11:22
Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions
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Cited by 29 •
2013
Neutrophil adherence to the activated endothelium at sites of infection is an integral component of the host's inflammatory response. Described in this report is a neutrophil binding assay that allows for the in vitro quantitation of primary human neutrophil binding to endothelial cells activated by inflammatory mediators under static conditions.
09:11
Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification
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Cited by 4 •
2015
Here, we present a protocol to continuously quantify cell adhesion and de-adhesion processes with high temporal resolution in a non-invasive manner by cell-substrate impedance and live cell imaging analyses. These approaches reveal the dynamics of cell adhesion/de-adhesion processes triggered by matrix modification and their temporal relationship to adhesion-dependent signaling events.
11:04
Isolation of Valvular Endothelial Cells
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Cited by 82 •
2010
We provide a method for isolating and culturing pure populations of heart valve endothelial cells (VEC). VEC can be isolated from either side of the cusp or leaflet and immediately following, underlying interstitial cell (VIC) isolation is straightforward.
FAQs
The behavior of endothelial cell adhesion depends on coordinated interactions among cadherins, integrins, and selectins rather than on a single adhesive signal. These molecules help connect endothelial cells with neighboring cells, the extracellular matrix, or circulating blood cells. Examining which interaction changes can therefore reveal whether vascular organization, barrier maintenance, or blood-cell contact is being affected.
Adhesion is not static: cytoskeletal forces, inflammatory signals, and changes in blood flow regulate how adhesion molecules bind. These influences can shift endothelial contacts or interactions with cells in circulation, making local conditions important to experimental interpretation. Accounting for them helps distinguish a change caused by molecular regulation from one associated with the physical or inflammatory environment.
In cancer research, altered endothelial adhesion can connect vascular barrier disruption with metastatic progression. Greater permeability may make the vessel wall more permissive, while adhesion between tumor cells and the endothelium can support their attachment and passage across that wall. Studying both effects clarifies how tumor-vascular interactions contribute to dissemination.
Studies should examine adhesion under changes in blood flow and in the presence of inflammatory signals, while also considering cytoskeletal forces. Researchers can then relate altered molecular binding to barrier function, vascular organization, or contact with circulating cells. This variable-focused approach is useful for separating baseline endothelial behavior from cancer-associated changes in the vessel environment.
Findings from these studies can inform several lines of cancer research. Changes in endothelial adhesion may help investigators examine angiogenesis, metastatic dissemination, and the interaction between tumors and blood vessels. The resulting observations can also identify whether altered vascular permeability or tumor-cell attachment is a relevant feature when considering therapies directed at tumor-vascular communication.
Vascular permeability and adhesion are related but not interchangeable readouts. Permeability reflects barrier leakiness, whereas adhesion concerns attachment among endothelial cells, the extracellular matrix, circulating blood cells, or tumor cells at the vessel interface. In cancer research, measuring both can help determine whether dissemination is associated mainly with barrier alteration, tumor attachment, or both.