Upright Droplet Technique
Upright Droplet Technique - Related Videos
09:47
Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis
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Cited by 13 •
2015
The ex vivo upright droplet culture is an alternative to current in vitro and in vivo experimental techniques. This protocol is easy to perform and requires smaller amounts of reagent, while permitting the ability to manipulate and study fetal vascularization, morphogenesis, and organogenesis.
07:34
Upright Imaging of Drosophila Embryos
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Cited by 7 •
2010
Here we present a mounting protocol for stained Drosophila embryos in an upright position that allows imaging of cross-sections using Confocal microscopy.
12:29
Upright Imaging of Drosophila Egg Chambers
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Cited by 1 •
2015
The upright imaging method described in this protocol allows for the detailed visualization of the poles of a developing Drosophila melanogaster egg. This end-on view provides a new perspective into the arrangements and morphologies of multiple cell types in the follicular epithelium.
03:37
Lipid Droplet Staining of Larval Drosophila Oenocytes: A Technique for Assessment of Lipid Droplet Accumulation Under Normal and Stressed Condition Using BODIPY-Based Dye
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2025
This video demonstrates lipid droplet staining in Drosophila larval oenocytes - hepatocyte-like cells - using the lipophilic fluorescent BODIPY based dye. These cells act as a model for studying lipid metabolism during development and in response to environmental stress such as starvation.
09:41
Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
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Cited by 15 •
2014
Two techniques for isolating cellular lipid droplets from 1) yeast cells and 2) human placentas are presented. The centerpiece of both procedures is density gradient centrifugation, where the resulting floating layer containing the droplets can be readily visualized by eye, extracted, and quantified by Western Blot analysis for purity.
FAQs
Surface tension helps the defined volume of culture medium retain a stable droplet shape on its support. This physical boundary keeps embryos or cells localized rather than dispersed, making the sample easier to observe and manipulate. Maintaining localization is especially useful when developmental changes must be followed repeatedly in the same optically accessible preparation.
A defined medium volume helps establish the compact experimental environment in which the sample remains positioned and visible. Consistent droplet conditions support controlled observation and make repeated measurements or parallel testing more comparable. The microscale format also limits reagent use, which is valuable when experiments examine several developmental conditions.
The method supports continuous visualization of growth, morphogenesis, and cell behavior. Because embryos or cells remain localized in an optically accessible droplet, investigators can monitor changes during development and relate visible structural or behavioral outcomes to the conditions being tested. This makes the technique relevant to experiments requiring repeated observation rather than a single endpoint.
Setup begins by placing a defined volume of culture medium on a support, allowing surface tension to preserve the droplet. Embryos or cells are then positioned within the droplet for observation, manipulation, or treatment. The preparation can subsequently be monitored during development, enabling repeated visualization while maintaining the sample in a compact format.
The essential elements are a support, a defined volume of culture medium, and the biological sample, such as embryos or cells. The preparation must also allow optical access for imaging and provide an arrangement compatible with manipulation or treatment when those operations are part of the experiment. These components support controlled developmental observation.
Researchers may choose this approach when they need precise environmental control, continuous visualization, or repeated measurements of developing samples. Its compact format is also suited to limiting reagent use and testing multiple developmental conditions in parallel. These features can simplify sample handling while preserving access to growth, morphogenesis, and cell-behavior outcomes.