Panicle Trimming
Panicle Trimming - Related Videos
01:10
Trimmed Mean
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2023
While measuring the mean of a data set, care needs to be taken when associating the mean to its central tendency. The same goes for the arithmetic mean, the geometric mean, or the harmonic mean. This is because the presence of a single outlier data value can significantly affect the mean. That is, the mean is sensitive to fluctuations in the data set. Although certain measures of central tendency are not sensitive to outliers, there are alternative versions of the mean that get around the...
08:35
Methods for Performing Crosses in Setaria viridis, a New Model System for the Grasses
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Cited by 31 •
2013
We have developed a methodology for performing crosses in Setaria viridis (S. viridis). The method involves pruning the panicle prior to a hot water treatment to kill viable pollen. Crosses are performed following a well-controlled growth regime and typically result in the recovery of 1 to 7 cross-pollinated seed/s per panicle.
09:46
A Method for Obtaining Serial Ultrathin Sections of Microorganisms in Transmission Electron Microscopy
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Cited by 15 •
2018
This study presents reliable and easy procedures for obtaining serial ultrathin sections of a microorganism without expensive equipment in transmission electron microscopy.
08:38
Targeted DNA Methylation Analysis by Next-generation Sequencing
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Cited by 83 •
2015
Bisulfite amplicon sequencing (BSAS) is a method for quantifying cytosine methylation in targeted genomic regions of interest. This method uses bisulfite conversion paired with PCR amplification of target regions prior to next-generation sequencing to produce absolute quantitation of DNA methylation at a base-specific level.
03:50
Preparing Free-Floating Slice Cultures from an Adult Human Brain
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2025
This video demonstrates a technique for preparing free-floating slice cultures from the adult human brain. Thin slices are generated from a brain section using a vibratome. These slices are then suspended in a culture medium supplemented with growth factors to maintain tissue viability.
FAQs
The main mechanism is reduced reproductive demand. When some flowering or grain-bearing units are removed, leaves continue producing assimilates, while fewer developing grains or seeds compete for photosynthates, water, and minerals. This change allows researchers to examine how resource availability affects grain filling, reproductive development, and eventual yield under defined environmental conditions.
The amount and location of removal determine which reproductive units remain as sinks for plant resources. Altering these features changes the distribution of flowers or grains within the panicle and can produce different effects on reproductive performance. Comparing contrasting trimming patterns therefore helps reveal how reproductive-unit number and position influence grain filling and yield.
Environmental conditions determine how much assimilate and other resources the plant can provide after reproductive demand has been reduced. Light affects assimilate production, while temperature and resource availability influence flowering and grain filling. Applying trimming under different conditions helps separate effects caused by reproductive-unit reduction from responses associated with the surrounding environment.
A basic experiment removes part or all of a plant’s panicle, with the extent and position of removal selected according to the research question. The manipulated plants are then evaluated for flowering, grain filling, or yield under the chosen environmental conditions. This controlled approach links a defined change in reproductive structure with measurable plant responses.
Including plants with different levels of panicle removal provides a basis for comparing reproductive outcomes as sink demand changes. Differences in flowering, grain filling, or yield can then be considered alongside light, temperature, and resource conditions. Such comparisons help determine whether environmental effects become stronger, weaker, or otherwise altered when fewer grains or seeds are developing.
The technique is useful when researchers need to test how environmental conditions influence reproductive performance rather than simply observe final yield. It supports studies of flowering, grain filling, and productivity under changing light, temperature, or resource availability. The resulting comparisons can improve experimental design and contribute to strategies for managing crop productivity in variable environments.