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Methods for Performing Crosses in Setaria viridis, a New Model System for the Grasses
 

Methods for Performing Crosses in Setaria viridis, a New Model System for the Grasses

Article doi: 10.3791/50527
October 1st, 2013

Summary October 1st, 2013

We have developed a methodology for performing crosses in Setaria viridis (S. viridis). The method involves pruning the panicle prior to a hot water treatment to kill viable pollen. Crosses are performed following a well-controlled growth regime and typically result in the recovery of 1 to 7 cross-pollinated seed/s per panicle.

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