Total Lipid Determination
Total Lipid Determination - Related Videos
14:59
Determination of Total Lipid and Lipid Classes in Marine Samples
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Cited by 3 •
2021
This protocol is for the determination of lipids in seawater and biological specimens. Lipids in filtrates are extracted with chloroform or mixtures of chloroform and methanol in the case of solids. Lipid classes are measured by rod thin-layer chromatography with flame ionization detection and their sum gives the total lipid content.
09:18
Purification of a Total Lipid Extract with Column Chromatography
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2023
Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst The product of an organic solvent extraction, a total lipid extract (TLE), is often a complex mixture of hundreds, if not thousands, of different compounds. The researcher is often only interested in a handful of compounds. The compounds of interest may belong to one of several classes of compounds, such as alkanes, ketones, alcohols, or acids (Figure 1), and it may be useful to remove the compound classes to which it does...
22:00
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
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Cited by 48 •
2010
Herein is described the procedure implemented in the Caffrey Membrane Structural and Functional Biology Group to set up manually crystallization trials of membrane proteins in lipidic mesophases.
10:52
Gramicidin-based Fluorescence Assay; for Determining Small Molecules Potential for Modifying Lipid Bilayer Properties
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Cited by 34 •
2010
We introduce a fast fluorescence-based assay that monitors the rate of fluorescence quenching as a measure of gramicidin channel activity. The gramicidin channels are used as molecular force transducers to monitor changes in lipid bilayer properties as sensed by bilayer spanning proteins.
10:59
Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy (FCS)
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Cited by 9 •
2012
A technique to probe the lipid raft partitioning of fluorescent proteins at the plasma membrane of living cells is described. It takes advantage of the disparity in diffusion times of proteins located inside or outside of lipid rafts. Acquisition can be performed dynamically in control conditions or after drug addition.
FAQs
The extraction relies on differences in solubility: lipid material transfers into the selected solvent, while many nonlipid components remain outside the extract. After separation, the collected extract represents the lipid-soluble fraction of the original sample. This principle allows the same analytical approach to be applied to biological material, sediment, water, and other environmental samples.
Solvent removal ensures that the measured residue reflects extracted lipid material rather than the mass or signal of the solvent itself. Once the solvent has been removed, the residue can be evaluated gravimetrically or by an instrumental method. This step directly affects the reliability of comparisons among samples, locations, and sampling times.
A total value summarizes the combined lipid content of a sample rather than emphasizing one lipid component. That integrated measurement can provide a broad indicator of organic matter and biological material across environmental samples. It is therefore useful when the study focuses on overall changes in sample composition, biomass, or lipid-associated partitioning rather than a single constituent.
A typical workflow begins with the environmental or biological sample, followed by solvent extraction to separate lipid-soluble material from nonlipid components. The extract is then collected, and the solvent is removed. Finally, the remaining lipid fraction is quantified gravimetrically or instrumentally, producing a result that can be compared across samples or conditions.
The method can be applied to biological samples, sediments, water, and other environmental materials. In aquatic or sediment studies, results can help characterize organic matter and biological material, while water and wastewater measurements can support evaluation of composition. The broad sample range makes the analysis useful across different environmental monitoring and research designs.
Researchers can compare lipid levels among locations or across time to evaluate ecosystem conditions and environmental change. Measurements may also help assess microbial or aquatic biomass, characterize sediment and wastewater composition, and examine lipid-associated contaminant partitioning. In treatment or remediation studies, changes in the measured levels can contribute to evaluating process performance.