Flare Conidia
Flare Conidia - Related Videos
02:22
Real-Time Assessment of the Antifungal Activity of Primary Human Immune Cells
0 Views •
2025
This video demonstrates live cell imaging for the analysis of antifungal activity in human neutrophils using fluorescent Aspergillus reporter conidia. Initially, both red and magenta fluorescence are detected within neutrophils, while red fluorescence decreases over time, indicating the degradation of conidia and confirming the antifungal activity of neutrophils.
09:43
Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry
0 Views •
Cited by 2 •
2019
This protocol provides a fast and reliable method to quantitatively measure phagocytosis of Aspergillus fumigatus conidia by human primary phagocytes using flow cytometry and to discriminate phagocytosis of conidia from mere adhesion to leukocytes.
12:29
Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus
0 Views •
Cited by 10 •
2017
Here, we describe a protocol to assess antifungal activity of primary human immune cells in real-time using fluorescent Aspergillus reporter conidia in conjunction with live-cell video microscopy and flow cytometry. Generated data provide insight into host cell-Aspergillus interactions such as fungicidal activity, phagocytosis, cell migration and inhibition of fungal growth.
07:21
Viability Assay of Trichoderma stromaticum Conidia Inside Human Peripheral Blood Mononuclear-Derived Macrophages
0 Views •
Cited by 1 •
2023
The technique involving the phagocytosis of fungal conidia by macrophages is widely used for studies evaluating the modulation of the immune responses against fungi. The purpose of this manuscript is to present a method for evaluating the phagocytosis and clearance abilities of human peripheral blood mononuclear-derived macrophages stimulated with Trichoderma stromaticum conidia.
15:01
Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung
0 Views •
Cited by 3 •
2021
We describe the method for quantitative analysis of the distribution of Aspergillus fumigatus conidia (3 µm in size) in the airways of mice. The method also can be used for the analysis of microparticles and nanoparticle agglomerate distribution in the airways in various pathological condition models.
FAQs
The stable fungal fluorescence signal serves as an identity reference for the conidia, while the second reporter supplies event-sensitive information. Because the two signals are read together, investigators can separate fungal presence from changes associated with host-cell encounter, internalization, or processing. This pairing improves interpretation of immune-cell interactions compared with relying on a single fluorescence measurement.
A change in the second reporter indicates that the conidia have undergone a host-cell-associated event, but its meaning depends on the event being examined. In the Flare Conidia design, signal behavior can be used to distinguish encounter, internalization, and processing. That distinction helps map where conidia are in the interaction rather than merely count fungal particles.
Their dual-label readout can be interpreted alongside the host-cell response to assess whether conidia remain viable during cellular interaction. This adds a fate-related dimension to measurements of phagocytosis: researchers can examine not only whether immune cells take up conidia, but also how conidial viability changes during that response.
Microscopy can show the relationship between conidia and host cells, whereas flow cytometry can quantify fluorescence patterns across measured events. With Flare Conidia, both approaches can use the stable fungal signal and changing reporter to analyze immune recognition, uptake, and processing, offering complementary views of the same host-pathogen interaction.
Researchers examine conidia after interaction with immune cells, using microscopy or flow cytometry to evaluate the paired fluorescence signals. The stable signal identifies fungal material, while the second reporter helps classify host-cell encounter or uptake-related states. These measurements can then quantify phagocytosis and compare conidial fate during the cellular response.
They are particularly useful when a study needs to connect immune recognition with what happens to fungal material inside or around host cells. The approach supports analysis of phagocytosis, intracellular fungal fate, and conidial viability in the same experimental framework, making it relevant to investigations of antifungal immunity and host-pathogen interactions.