In Vivo Transfer
In Vivo Transfer - Related Videos
22:02
Gene Transfer to the Developing Mouse Inner Ear by In Vivo Electroporation
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Cited by 23 •
2012
The mouse inner ear is a placode-derived sensory organ whose developmental program is elaborated during gestation. We define an in utero gene transfer technique consisting of three steps: mouse ventral laparotomy, transuterine microinjection, and in vivo electroporation. We use digital video microscopy to demonstrate the critical experimental embryological techniques.
05:40
In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation
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Cited by 4 •
2016
Here, we present an in vivo technique for gene transfer to Schwann cells (SCs) in the rodent sciatic nerve. This simple technique is useful for investigating signaling mechanisms involved in the development and maintenance of myelinating SCs.
10:18
In Vivo Gene Transfer to the Rabbit Common Carotid Artery Endothelium
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Cited by 8 •
2018
This method is to introduce a transgene into the endothelium of rabbit carotid arteries. Introduction of the transgene allows the assessment of the biological role of the transgene product either in normal arteries or disease models. The method is also useful for measuring activity of DNA regulatory sequences.
09:16
Simultaneous ex vivo Functional Testing of Two Retinas by in vivo Electroretinogram System
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Cited by 36 •
2015
Ex vivo ERG can be used to record electrical activity of retinal cells directly from isolated intact retinas of animals or humans. We demonstrate here how common in vivo ERG systems can be adapted for ex vivo ERG recordings in order to dissect the electrical activity of retinal cells.
10:05
In vivo 19F MRI for Cell Tracking
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Cited by 21 •
2013
We describe a general protocol for in vivo cell tracking using MRI in a mouse model with ex vivo labeled cells. A typical protocol for cell labeling, image acquisition processing and quantification is included.
FAQs
In vivo transfer can separate cell-intrinsic immune functions from effects imposed by the host. Investigators place defined donor immune cells into a recipient and examine whether their behavior persists, changes, or depends on surrounding signals. Comparing donor-cell responses within the living recipient helps reveal how circulation, tissue migration, antigen exposure, or infection shape immune activity.
Recipient tissues provide more than a destination: they supply the physiological setting in which transferred cells encounter host signals. As cells circulate, migrate, and potentially expand, their distribution and responses can indicate how the environment supports or constrains them. This is especially useful when interpreting immune behavior that cannot be understood from donor cells alone.
Exposure to antigen or infection gives the transferred population a functional challenge. Researchers can then assess whether donor cells respond in the recipient and relate that response to immune protection or disease-related effects. The same design can help examine immune memory, because transferred cells are evaluated for behavior and activity after encountering relevant stimulation in vivo.
A typical study begins by selecting defined donor immune cells or another material of interest, introducing it into a living recipient, and then examining its behavior. The analysis follows whether the transferred component circulates, reaches tissues, expands, and responds to antigen or infection. This sequence connects the introduced material with physiological outcomes in the host.
Study outcomes can include the transferred cells’ distribution, expansion, and responses, together with effects on the host. In immunology, investigators may use these observations to assess immune protection, pathology, or therapeutic potential. Examining both cellular behavior and organism-level effects helps connect a defined immune component with beneficial or harmful consequences.
Applications extend across lymphocyte development, immune memory, host-pathogen interactions, and disease mechanisms. The method is valuable in immunology and infection because it places a defined immune population or infectious material into a physiological context, allowing researchers to connect cellular behavior with protection, pathology, or changes associated with infection and disease.