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Gene Transfer to the Developing Mouse Inner Ear by In Vivo Electroporation
 

Gene Transfer to the Developing Mouse Inner Ear by In Vivo Electroporation

Article doi: 10.3791/3653
June 30th, 2012

Summary June 30th, 2012

The mouse inner ear is a placode-derived sensory organ whose developmental program is elaborated during gestation. We define an in utero gene transfer technique consisting of three steps: mouse ventral laparotomy, transuterine microinjection, and in vivo electroporation. We use digital video microscopy to demonstrate the critical experimental embryological techniques.

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