Murine Wound Model
Murine Wound Model - Related Videos
05:39
Murine Model of Wound Healing
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Cited by 207 •
2013
A murine model of cutaneous wound healing that can be used to assess therapeutic compounds in physiological and pathophysiological settings.
02:48
Colonoscopic Guided Murine Wound Model: A Method to Isolate Biopsy Induced Wound Bed from Colon to Understand the Healing Process
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2025
In this video, we describe a procedure to isolate the colonoscopic-induced wound bed at an appropriate time point for detailed molecular analysis. This analysis helps to understand the wound healing process in inflammatory conditions of the GI tract.
08:35
Demonstration of the Rat Ischemic Skin Wound Model
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Cited by 32 •
2015
The rat, due to its size, availability, and rather docile behavior, has been utilized as a research model for many years. The goal of this protocol is to utilize the rat as an ischemic skin wound healing model to provide valuable insight into the pathophysiology of chronic wounds.
06:36
Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis
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Cited by 27 •
2020
This protocol describes how to generate bilateral, full-thickness excisional wounds in mice and how to subsequently monitor, harvest, and prepare the wounds for morphometric analysis. Included is an in-depth description of how to use serial histological sections to define, precisely quantify and detect morphometric defects.
05:00
Wound Assay to Evaluate the Migratory Capacity of Murine Myoblasts in Co-Culture
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2025
This video describes a non-contact co-culture wound healing assay to evaluate the migratory capacity of murine myoblasts. This helps to understand signal-sending and signal-receiving components of the paracrine non-canonical Wnt signaling interactions in vitro.
FAQs
A useful analysis connects immune recruitment and cytokine signaling with tissue-level repair. Investigators can compare the timing and extent of inflammation, re-epithelialization, and angiogenesis rather than treating wound closure as a single outcome. This integrated view helps identify whether delayed healing reflects altered immune regulation, impaired tissue restoration, or interactions between both processes.
Adding infection allows researchers to examine host-pathogen interactions alongside repair. Changes in bacterial burden over time can be interpreted with immune-cell recruitment, cytokine signaling, and healing measurements to determine how microbial persistence relates to inflammation or delayed tissue restoration. This design supports investigation of infection-associated mechanisms rather than evaluating wound closure in isolation.
Genetically modified animals can reveal whether a specific biological factor contributes to inflammation, repair, or infection control by comparing their responses with appropriate control animals. Differences in cytokine signaling, immune-cell recruitment, re-epithelialization, angiogenesis, or bacterial burden can link that factor to a measurable outcome and clarify its role in host defense or tissue recovery.
Treatment comparisons show whether an intervention changes the course of repair or infection-related responses. Researchers may examine effects on inflammatory signaling, immune-cell recruitment, tissue restoration, angiogenesis, and bacterial burden over time. Interpreting several outcomes together helps distinguish an intervention that improves healing from one that mainly alters inflammation or microbial control.
The workflow begins with laboratory mice receiving controlled, standardized skin wounds, followed by observation at defined stages of the response. Researchers track repair-associated processes, immune and cytokine responses, and, when infection is included, bacterial burden over time. Comparing animals by genotype or treatment then identifies changes in healing or host-pathogen interactions.
This model is useful when investigators need to test antimicrobial therapies, immunomodulators, or other treatments in the setting of tissue injury. Outcomes can indicate whether an intervention influences bacterial control, inflammatory responses, or restoration of the wound environment. Such evidence supports mechanistic research and can help evaluate factors relevant to delayed healing in human disease.