Necrosis Assay
Necrosis Assay - Related Videos
02:12
Muscle Necrosis Assay in C. elegans Induced by a Pathogenic Aeromonas Strain
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2026
Source: Chen, Y. et. al., Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model. J. Vis. Exp. (2018)This video demonstrates a muscle necrosis assay in C. elegans (Caenorhabditis elegans) using pathogenic Aeromonas strains. By feeding green fluorescent protein (GFP)-expressing worms on Aeromonas-seeded plates, the assay models bacterial infection and reveals tissue damage through fluorescence imaging. The progressive loss of muscle structure and GFP...
01:16
Necrosis
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2023
Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”. Morphological Manifestations of Necrosis Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become anucleated and die, but their...
01:16
Cellular Injury IV: Necrosis
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2026
Necrosis is a form of irreversible cell death caused by severe injury such as ischemia, toxins, or trauma. Unlike programmed cell death, it is an uncontrolled, pathological process that typically provokes inflammation in surrounding tissues.Pathophysiologic ChangesNecrosis begins when cells sustain critical damage, leading to swelling of organelles, particularly mitochondria, and rapid ATP depletion. As energy levels decline, membrane ion pumps fail, leading to calcium influx and eventually,...
04:37
Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation
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Cited by 1 •
2025
The present protocol provides a comprehensive set of procedures necessary to analyze procoagulant platelets, which exhibit overlapping features of necrosis, apoptosis, and platelet activation.
09:38
An Assay for Lateral Line Regeneration in Adult Zebrafish
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Cited by 11 •
2014
Because many zebrafish models of neurological and non-neurological diseases are studied in the adult fish rather than the embryo/larvae, we developed a quantitative lateral line regenerative assay that can be applied to adult zebrafish disease models. The assay involved resolution at the 1) neuromast and 2) individual hair cell levels.
FAQs
Propidium iodide and LDH report membrane damage through different physical outputs. Once necrotic injury compromises the plasma membrane, propidium iodide can enter cells that normally exclude it, whereas LDH appears in the surrounding medium after intracellular contents escape. Measuring either signal converts loss of integrity into a quantitative readout for comparing cellular injury across experimental conditions.
Using both readouts can distinguish how necrotic injury is represented in an experiment without changing the underlying event being assessed. Propidium iodide emphasizes entry into membrane-compromised cells, while LDH measurement examines material released outside the cells. This distinction matters when interpreting whether the result is expressed through affected cells or extracellular release.
In infection and immune-cell experiments, the measured injury reflects the effect of the tested biological interaction on target-cell membrane integrity. A higher necrosis-assay result can support evidence of pathogen-induced cytotoxicity or immune-cell-mediated damage, but the readout should be interpreted in relation to the experimental condition rather than treated as a direct measure of pathogen burden or immune protection.
A basic workflow begins by exposing the relevant cells or infected targets to the condition being studied, then measuring membrane permeabilization or released LDH. The investigator can quantify propidium iodide entry into cells or analyze LDH in the surrounding medium. Aligning the selected readout with the biological question allows comparison of injury among experimental conditions.
Necrosis assays support comparisons of virulence by showing how strongly different infectious conditions damage cells. They also reveal differences in host-cell susceptibility, because target populations can show different degrees of membrane injury under comparable study conditions. These measurements connect cellular damage to infection outcomes and help identify conditions associated with greater or lesser cytotoxicity.
In immunology, the method helps examine whether immune-cell activity is associated with target-cell injury during infection-related experiments. Results can contribute to evaluating protective or harmful inflammatory responses when interpreted alongside the infected or target-cell context. The assay therefore links a measurable cellular outcome, membrane damage, with broader questions about host defense and tissue injury.