T-cell Depletion
T-cell Depletion - Related Videos
06:17
Depletion of Specific Cell Populations by Complement Depletion
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Cited by 15 •
2010
To effectively study the function of immune cell populations their purification is often required. Complement depletion is a fast and inexpensive technique for the isolation of immune cell populations with high purity.
07:55
Ground State Depletion Super-resolution Imaging in Mammalian Cells
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Cited by 16 •
2017
This article outlines a protocol for the detection of one or more plasma and/or intracellular membrane proteins using Ground State Depletion (GSD) super-resolution microscopy in mammalian cells. Here, we discuss the benefits and considerations of using such approaches for the visualization and quantification of cellular proteins.
07:10
Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells
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Cited by 9 •
2016
Human tumor xenografts are vascularized and infiltrated by cells of mouse origin during the growth phase in vivo. To circumvent the bias caused by these contaminating cells during downstream analysis, we developed a method allowing for comprehensive depletion of all mouse cells by magnetic cell sorting.
08:13
Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60
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Cited by 3 •
2017
Here, we present the real-time monitoring of cell migration in a wound-healing assay using TIP60-depleted MCF10A breast epithelial cells. The implementation of live-cell imaging techniques in our protocol allows us to analyze and visualize single-cell movement in real time and across time.
08:50
Depletion and Reconstitution of Macrophages in Mice
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Cited by 118 •
2012
Macrophages play a central role in homeostasis and pathology in many tissues. The protocol presented here describes methods for depleting macrophages in vivo, deriving polarized macrophages from bone marrow aspirates, and adoptively transferring macrophages into mice. These techniques allow determination of the role that polarized macrophages play in health and disease.
FAQs
The biological consequence depends on how T cells are lost. Antibody-mediated killing and complement activation use immune-directed mechanisms, whereas targeted drug treatment applies a pharmacologic approach. Infection-driven loss reflects a disease process rather than an experimentally imposed intervention. Comparing these mechanisms helps investigators distinguish effects caused by T-cell absence from effects associated with the depletion method itself.
Removing T cells from blood, tissues, or a biological sample can produce different experimental interpretations because these compartments represent different parts of the immune system. A blood-based reduction may not describe tissue-associated cells, while depletion in a sample may have a narrower scope. Identifying the compartment is therefore essential when relating depletion to adaptive immune responses.
T-cell depletion can change the balance and strength of adaptive immunity by reducing the lymphocyte population responsible for relevant immune functions. In infection studies, this alteration can affect pathogen clearance, immune regulation, or immunopathology. The resulting phenotype must be interpreted in relation to the depletion context, because loss of T cells may influence protection and harmful inflammation in different ways.
Controlled depletion is introduced deliberately so researchers can examine the consequences of reducing T cells under a defined experimental or therapeutic context. Infection-driven loss occurs as part of disease biology and may coincide with changes caused by the infection itself. This distinction matters because controlled depletion supports causal analysis, whereas infection-associated loss reflects a more complex biological process.
Researchers use controlled depletion to test whether T cells contribute to removing a pathogen from the host. They compare immune or infection outcomes in settings with reduced T-cell participation and then assess consequences for pathogen clearance, immune regulation, and immunopathology. This approach helps separate T-cell-dependent protection from immune effects that arise through other processes.
In transplantation, reducing T-cell activity or numbers can help address immune reactions against the graft. The rationale is to lessen graft rejection, an immune response directed toward transplanted tissue. T-cell depletion therefore serves as a way to study how adaptive immunity contributes to transplant outcomes and to evaluate strategies intended to improve graft acceptance.
Cellular therapy research uses T-cell depletion to examine or reduce immune reactions associated with transferred cells. By limiting T-cell participation, investigators can study conditions relevant to graft-versus-host disease and assess how immune composition affects treatment outcomes. Its value lies in connecting the presence of T cells with unwanted responses during therapeutic cell transfer.
Monitoring after depletion reveals whether the immune system is recovering and whether reduced T-cell availability is associated with greater susceptibility to infection. These measurements provide context for interpreting treatment or experimental outcomes rather than treating depletion as a permanent endpoint. In immunology and infection research, they also connect cellular changes with later immune function.