Corneal Endothelium Transplantation
Corneal Endothelium Transplantation - Related Videos
09:59
An “All-laser” Endothelial Transplant
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Cited by 5 •
2015
An all laser procedure is proposed in the endothelial transplant. The surgical technique is based on the use of a femtosecond laser to prepare the donor tissue. Laser welding technique is then used to secure the donor endothelium in the correct position.
08:37
Corneal Donor Tissue Preparation for Endothelial Keratoplasty
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Cited by 31 •
2012
Endothelial corneal transplantation is a surgical technique for treatment of posterior corneal diseases. Mechanical microkeratome dissection to prepare tissue results in thinner, more symmetric grafts with less endothelial cell loss and improved outcomes. Dissections can be performed at the eye bank prior to corneal transplantation surgery.
09:03
Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation
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Cited by 10 •
2014
Mice have been used as a model for studying many forms of transplantation, including corneal transplantation. We describe in this report a murine model for both acute and late-term corneal transplantation.
04:11
Laser-assisted Selective Ablation of Porcine Corneal Endothelial Cells: A Technique to Induce Focused Porcine Corneal Endothelial Injury for Ex Vivo Studies
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2025
This video demonstrates a non-invasive laser-assisted procedure to selectively damage the corneal endothelial cells in the porcine eye. The developed ex vivo model of endothelial cell damage can be used to study diseases of the corneal endothelium.
06:20
Imaging Leukocyte Adhesion to the Vascular Endothelium at High Intraluminal Pressure
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Cited by 22 •
2011
This is a method to visualise leukocyte adhesion to the endothelium in harvested pressurised vessels. The technique enables studying vascular adhesion under shear flow with differing intraluminal pressures up to 200 mmHg thus mimic-ing the pathophysiological conditions of high blood pressure.
FAQs
Endothelial ion pumps maintain corneal clarity by removing excess fluid from the cornea’s stromal tissue. This fluid regulation, called stromal deturgescence, helps preserve the optical condition needed for transparent corneal tissue. After a donor endothelial layer is positioned on the posterior cornea, its pumping activity is therefore central to the procedure’s intended visual benefit.
Selective replacement targets the diseased or damaged endothelial component rather than replacing the entire cornea. Preserving much of the patient’s existing corneal tissue is a defining surgical advantage of this approach and helps explain why corneal endothelium transplantation can support faster visual recovery. The strategy also provides a basis for studying focused tissue repair.
DMEK and DSAEK are named procedures within corneal endothelium transplantation that use a thin donor layer placed on the posterior cornea. The overview identifies both as ways to replace failing endothelial tissue while retaining much of the patient’s cornea. Their shared relevance is selective restoration of the endothelial function required for corneal transparency and vision.
Endothelial failure is the clinical problem addressed by this transplant approach. The overview specifically identifies Fuchs endothelial corneal dystrophy and bullous keratopathy as conditions treated with procedures such as DMEK and DSAEK. In these settings, replacing the affected endothelial layer aims to restore fluid control in the cornea and improve transparency and vision.
During the procedure, a thin donor layer is positioned on the posterior surface of the cornea. This placement brings donor endothelial cells into the location where they can perform fluid-removing ion-pump activity. The workflow is therefore anatomically selective: it focuses donor tissue at the corneal interface responsible for maintaining stromal deturgescence.
The intended outcome is restoration of corneal transparency and vision, supported by renewed endothelial control of excess stromal fluid. The overview also associates selective tissue replacement with faster visual recovery. These outcomes explain the method’s clinical value when endothelial dysfunction is the source of visual impairment and the cornea requires restoration of its fluid-regulating function.
Corneal endothelium transplantation offers a model for linking targeted tissue replacement with restoration of a specific cell function. Because the transplanted endothelial layer must maintain stromal deturgescence through ion pumps, the approach highlights the importance of functional cell replacement, not merely structural coverage. This principle informs advances in cell-based regenerative therapies.