Porcine Aortic Endothelial Cells
Porcine Aortic Endothelial Cells - Related Videos
04:50
Porcine Aortic Endothelial Cell (pAEC) Isolation: An Enzyme-based Method to Isolate Endothelial Cells from Harvested Porcine Abdominal Aorta
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2025
In this video, we demonstrate the isolation of endothelial cells from the abdominal aorta of a pig model using one-step collagenase digestion. The endothelial cells can be cultured for further downstream applications.
08:20
Isolation and Primary Culture of Mouse Aortic Endothelial Cells
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Cited by 75 •
2016
The vascular endothelial cells play a significant role in many important cardiovascular disorders. This article describes a simple method to isolate and expand endothelial cells from the mouse aorta without using any special equipment. Our protocol provides an effective means of identifying mechanisms in endothelial cell physiopathology.
04:11
Laser-assisted Selective Ablation of Porcine Corneal Endothelial Cells: A Technique to Induce Focused Porcine Corneal Endothelial Injury for Ex Vivo Studies
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2025
This video demonstrates a non-invasive laser-assisted procedure to selectively damage the corneal endothelial cells in the porcine eye. The developed ex vivo model of endothelial cell damage can be used to study diseases of the corneal endothelium.
06:14
Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function
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Cited by 2 •
2017
Restenosis following cardiovascular procedures (bypass surgery, angioplasty, or stenting) is a significant problem reducing the durability of these procedures. An ideal therapy would inhibit smooth muscle cell (VSMC) proliferation while promoting regeneration of the endothelium. We describe a model for simultaneous assessment of VSMC proliferation and endothelial function in vivo.
09:23
Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells
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Cited by 31 •
2017
The aim of the protocol is to present an optimized procedure for the establishment of an in vitro blood-brain barrier (BBB) model based on primary porcine brain endothelial cells (pBECs). The model shows high reproducibility, high tightness, and is suitable for studies of transport and intracellular trafficking in drug discovery.
FAQs
Junctions between neighboring endothelial cells regulate how readily substances pass across the cultured monolayer. Changes in junctional behavior therefore provide an experimental readout of barrier permeability and vascular integrity. By examining this response after controlled stimulation, researchers can investigate mechanisms relevant to vascular injury and determine whether chemical exposure or other conditions disrupt endothelial barrier function.
Shear stress acts as a physical stimulus that can alter endothelial signaling pathways in culture. Those pathways influence vascular tone, inflammation, and coagulation, allowing investigators to examine how mechanical conditions shape vascular behavior. This makes the cells useful for connecting environmental forces acting on blood vessels with cellular responses involved in cardiovascular disease.
Chemical exposure can be used to examine how endothelial cells adjust signaling pathways that regulate barrier behavior, vascular tone, inflammation, and coagulation. Comparing responses under controlled laboratory conditions helps distinguish direct cellular effects from broader vascular outcomes. The approach is particularly relevant when studying potential drug effects or cellular reactions associated with vascular injury.
These processes represent interconnected aspects of endothelial function rather than isolated cellular events. Signaling changes in the cultured cells can provide insight into how vascular regulation, inflammatory activity, and coagulation-related behavior respond to defined stimuli. Studying them together helps medicine researchers relate cellular mechanisms to complex cardiovascular conditions and evaluate how experimental exposures may affect vascular health.
Investigators culture the cells as adherent monolayers and then examine their responses to selected conditions, including shear stress or chemical exposure. This format provides a controlled setting for studying barrier permeability and endothelial signaling without the complexity of an entire organism. The resulting observations can support experiments on vascular injury, drug effects, and material interactions.
The model supports studies of vascular injury, atherosclerosis, biomaterial compatibility, and drug effects. Researchers can use the monolayer system to examine barrier behavior and signaling responses under defined experimental conditions. These applications make the cells useful for testing how vascular lining cells respond to disease-related stimuli, therapeutic candidates, or materials intended for contact with the vascular system.
These experiments connect cellular responses with broader cardiovascular disease mechanisms and therapeutic development. Changes in permeability or signaling can be studied in relation to vascular tone, inflammation, coagulation, and injury. Because the culture environment is controlled, researchers can isolate particular stimuli and generate mechanistic evidence that helps interpret disease processes or assess potential treatment effects.