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Medicine

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Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells
 

Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells

Article DOI: 10.3791/56277-v 09:23 min September 24th, 2017
September 24th, 2017

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Summary

The aim of the protocol is to present an optimized procedure for the establishment of an in vitro blood-brain barrier (BBB) model based on primary porcine brain endothelial cells (pBECs). The model shows high reproducibility, high tightness, and is suitable for studies of transport and intracellular trafficking in drug discovery.

Tags

In Vitro Blood-brain Barrier Model Porcine Brain Endothelial Cells Drug Delivery Permeability Receptor Trafficking TEER Polarity Brain Isolation Meninge Removal Gray Matter DMEM/F-12 Medium Tissue Homogenizer
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