Rodent Kidney Assessment
Rodent Kidney Assessment - Related Videos
07:35
Use of Ultra-high Field MRI in Small Rodent Models of Polycystic Kidney Disease for In Vivo Phenotyping and Drug Monitoring
0 Views •
Cited by 14 •
2015
The use of ultra-high field MRI as a non-invasive way to obtain phenotypic information of rodent models for polycystic kidney disease and to monitor interventions is described. Compared with the traditional histological approach, MRI images can be acquired in vivo, allowing for longitudinal follow-up.
07:18
Transcutaneous Assessment of Renal Function in Conscious Rodents
0 Views •
Cited by 34 •
2016
Determination of glomerular filtration rate (GFR) is the gold standard to assess overall kidney function. However, traditional procedures to measure this parameter are cumbersome and require a large investment of time. Here we describe a faster and minimally invasive method to determinate GFR transcutaneously.
08:19
The Mouse Isolated Perfused Kidney Technique
0 Views •
Cited by 24 •
2016
The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.
08:50
Assessment of Vascular Function in Patients With Chronic Kidney Disease
0 Views •
Cited by 25 •
2014
The degree of vascular dysfunction and contributing physiological mechanisms can be assessed in patients with chronic kidney disease by measuring brachial artery flow-mediated dilation, aortic pulse-wave velocity, and vascular endothelial cell protein expression.
03:00
Extracting Intact Kidney from Mouse: A Technique to Obtain Kidney Without Renal Capsule from Murine Models
0 Views •
2023
In this video, we describe the procedure for kidney extraction from the retroperitoneal space of the mouse. The extracted kidney can further be microdissected to obtain an intact kidney without a renal capsule and the surrounding fat layer.
FAQs
Serum creatinine and blood urea nitrogen provide physiological indicators of renal performance, particularly impaired filtration. Examining both measurements gives investigators evidence of functional change rather than relying on tissue appearance alone. Their results become more informative when interpreted with urinalysis and histopathology, allowing functional abnormalities to be considered alongside altered concentrating ability, inflammation, or structural damage.
Urinalysis contributes information that serum creatinine and blood urea nitrogen cannot provide by themselves. It can reveal changes associated with the kidney’s concentrating ability, adding a different functional perspective to the assessment. Combining these findings with organ examination and tissue histopathology helps investigators characterize renal effects more completely and reduces reliance on a single outcome.
Histopathology provides tissue-level evidence of renal injury, including inflammation and damage that may not be fully represented by physiological measurements. This cellular and structural perspective helps connect observed kidney dysfunction with underlying tissue changes. In medicine-focused research, that connection supports interpretation of molecular and cellular findings in relation to broader renal disease mechanisms.
A standardized evaluation should combine serum creatinine and blood urea nitrogen measurements, urinalysis, organ examination, and histopathology. Each component addresses a different aspect of renal status, including filtration, concentrating ability, visible organ condition, and tissue injury. Using these complementary assessments within a consistent study framework improves reproducibility and supports clearer comparison across preclinical investigations.
Organ examination adds structural context to physiological and urinary results. When investigators assess the kidney as an organ, they can relate functional changes to evidence obtained from tissue analysis and other measurements. This integrated approach helps distinguish a broad pattern of renal abnormality from an isolated laboratory finding and supports more complete characterization of kidney health or injury.
In drug research, investigators use this assessment to characterize potential nephrotoxicity and to examine nephroprotective effects. Blood measurements, urinalysis, organ examination, and histopathology together show whether a treatment is associated with impaired renal function or tissue damage, or whether it may protect against renal injury. These findings contribute to preclinical evaluation before therapies advance to human research.
Rodent kidney assessment helps connect molecular and cellular observations with clinically relevant renal outcomes. By combining functional measurements with urinary, organ-level, and tissue-level evidence, investigators can study mechanisms of renal disorders in a controlled preclinical model. The resulting profile supports disease characterization and helps determine whether experimental findings warrant further development toward human research.