Cortical Area Transfection
Cortical Area Transfection - Related Videos
07:28
An Introduction to Transfection
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2023
Transfection is the process of inserting genetic material, such as DNA and double stranded RNA, into mammalian cells. The insertion of DNA into a cell enables the expression, or production, of proteins using the cells own machinery, whereas insertion of RNA into a cell is used to down-regulate the production of a specific protein by stopping translation. While the site of action for transfected RNA is the cytoplasm, DNA must be transported to the nucleus for effective transfection. There, the...
08:04
Neuronal Transfection Methods
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2023
Transfection - the process of transferring genetic material into cells - is a powerful tool for the rapid and efficient manipulation of gene expression in cells. Because this method can be used to silence the expression of specific proteins or to drive the expression of foreign or modified proteins, transfection is an extremely useful tool in the study of the cellular and molecular processes that govern neuron function. However, mature neurons have a number of properties that make them...
09:30
Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
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Cited by 49 •
2011
A gene transfer method into the developing mouse brain is described by using a unique surgical method and special shape of electrodes. This unique technique allows transfection of plasmid DNA temporally and spatially, which will aid many neuroscientists in studying brain development.
04:21
Protein Transfection of Mouse Lung
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Cited by 1 •
2013
Transgenic mice or viral vectors have been used to increase protein expression within the lung. However, these techniques are time-consuming, technically challenging and have off-target effects that can confound results. Our protein transfection protocol uses a lipid based transfection reagent and an ultrafine microsprayer to uniformly deliver active protein to lung cells.
15:40
Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons
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Cited by 63 •
2011
This protocol outlines the steps required to dissect, transfect via electroporation and culture mouse hippocampal and cortical neurons. Short-term cultures may be used for studies of axon outgrowth and guidance, while long-term cultures can be used for studies of synaptogenesis and dendritic spine analysis.
FAQs
The sequence and design of the delivered genetic material determine whether transfected cells produce a particular protein or show reduced expression of a target gene. DNA or RNA therefore serves different experimental purposes, depending on the molecular change being studied. Selecting the appropriate cargo links the intended genetic manipulation to measurable cellular, circuit, or behavioral outcomes.
DNA can provide instructions that drive production of a protein, whereas RNA can be designed to reduce expression of a target gene. This distinction allows researchers to either introduce a molecular function or suppress one. The choice affects how investigators interpret changes in neuronal activity, cellular properties, circuit organization, or behavior.
Restricting the manipulation to a defined cortical region helps connect a molecular change with the neural population and pathways located in that area. This spatial precision reduces ambiguity when interpreting results, because observed cellular or behavioral effects can be related more directly to the targeted cortical site rather than to broad, untargeted changes.
A conceptual workflow includes selecting DNA or RNA with the desired sequence, directing that cargo to a defined cortical area, enabling it to cross cell membranes, and assessing the resulting molecular or neural effects. The final analysis may examine protein production, reduced target-gene expression, localized labeling, pathway manipulation, or associated behavioral outcomes.
Researchers can apply this approach when they need to connect gene expression with neuronal function, circuit organization, development, or disease mechanisms. Localized genetic manipulation is especially useful when a question concerns a particular cortical region or neural population. The resulting changes can be evaluated across molecular, cellular, circuit, and behavioral levels.
Depending on the cargo and experimental design, outcomes may include production of a selected protein, reduced expression of a target gene, localized labeling, or manipulation of neural pathways. These molecular effects can then be related to changes in neuronal function, cortical circuit organization, development, disease-related processes, or behavior.