Microliter Syringe

A microliter syringe is a precision instrument designed to measure and deliver very small liquid volumes, typically at the microliter scale, with accuracy that supports controlled laboratory experiments. It uses a calibrated barrel, low-dead-volume plunger, and fine needle to create and dispense a defined volume while minimizing leakage, evaporation, and sample loss. In neuroscience, researchers use microliter syringes to administer drugs, tracers, or other solutions into targeted tissues, prepare samples for analysis, and support microinjection or infusion procedures. Their precision improves dose control, experimental reproducibility, and the study of neural circuits, pharmacological responses, and brain function.

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JoVE EoE - Bacterial Growth and Techniques

Extraction of Apoplastic Bacteria Using a Syringe-Based Pressure Method

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2025

Source: Rufián, J. S., et al, Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022)This video demonstrates a syringe-based pressure method for extracting apoplastic bacteria, which proliferate and form microcolonies within the apoplast, the intercellular space between plant cells. The method yields debris-free bacterial samples suitable for downstream single-cell analysis.

Research

JoVE Journal - Bioengineering
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Syringe-injectable Mesh Electronics for Stable Chronic Rodent Electrophysiology

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Cited by 31 •

2018

Mesh electronics probes seamlessly integrate and provide stable, long-term, single-neuron level recording within the brain. This protocol uses mesh electronics for in vivo experiments, involving the fabrication of mesh electronics, loading into needles, stereotaxic injection, input/output interfacing, recording experiments, and histology of tissue containing mesh probes.

Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle

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Cited by 2 •

2021

Here we demonstrate a method to apply fluid shear stress to cancer cells in suspension to model the effects of hemodynamic stress on circulating tumor cells.

Automated and High-throughput Microbial Monoclonal Cultivation and Picking Using the Single-cell Microliter-droplet Culture Omics System

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2025

This protocol describes how to use the Single-cell Microliter-droplet Culture Omics System (MISS cell) to perform microbial monoclonal isolation, cultivation, and picking. The MISS cell achieves an integrated workflow based on droplet microfluidic technology, which offers excellent droplet monodispersity, high parallel cultivation, and high-throughput biomass detection.

Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications

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Cited by 3 •

2018

Here we present a protocol to construct a pressure-controlled syringe pump to be used in microfluidic applications. This syringe pump is made from an additively manufactured body, off-the-shelf hardware, and open-source electronics. The resulting system is low-cost, straightforward to build, and delivers well-regulated fluid flow to enable rapid microfluidic research.

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