Neurofilament 200
Neurofilament 200 - Related Videos
04:33
Fluorescence Photoactivation Imaging of Neurofilament Transport in Myelinated Mouse Axons
0 Views •
2025
Source: Boyer, N. P. et. al., Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve. J. Vis. Exp. (2020) This video demonstrates the fluorescence photoactivation method to analyze neurofilament transport in myelinated axons of peripheral nerves. By tracking photoactivated green fluorophore-tagged neurofilaments, the technique provides insights into axonal transport in neurodegenerative diseases.
09:52
Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve
0 Views •
Cited by 2 •
2020
We describe fluorescence photoactivation methods to analyze the axonal transport of neurofilaments in single myelinated axons of peripheral nerves from transgenic mice that express a photoactivatable neurofilament protein.
09:49
Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
0 Views •
Cited by 13 •
2022
High-resolution cryo-EM maps of macromolecules can be also achieved by using 200 kV TEM microscopes. This protocol shows the best practices for setting accurate optics alignments, data acquisition schemes, and selection of imaging areas that are all essential for the successful collection of high-resolution datasets using a 200 kV TEM.
11:27
Selective Tracing of Auditory Fibers in the Avian Embryonic Vestibulocochlear Nerve
0 Views •
Cited by 3 •
2013
Here we describe a microdissection technique followed by fluorescent dye injection into the acoustic ganglion of early chick embryos for selective tracing of auditory axon fibers in the nerve and hindbrain.
01:30
Ultrastructural Analysis of Demyelination in Mouse Spinal Cord Cross-Sections
0 Views •
2025
The disease triggers autoreactive immune cells to attack myelin proteins, degrading the myelin sheath surrounding neuronal axons.
FAQs
Phosphorylation of Neurofilament 200, particularly within its C-terminal domains, helps organize neurofilament networks and regulate their interactions with other axonal components. This modification therefore affects how the filament system is arranged within the axon rather than simply marking the protein for detection. Studying phosphorylation can help relate cytoskeletal organization to axonal structure.
Neurofilament 200 operates as part of a neurofilament system rather than as an isolated structural element. Its assembly with other neurofilament proteins contributes to the organization of intermediate filament networks inside neurons. Examining this coordinated arrangement is important when interpreting changes in axonal architecture, because altered organization may reflect disruption of the broader filament network.
Changes in neurofilament organization can indicate that the internal architecture supporting the axon has been altered. Because these filaments contribute to axonal structure and caliber, their disorganization provides information about cytoskeletal changes within neurons. This makes Neurofilament 200 useful for investigating how neuronal structure is maintained or disturbed during development and damage.
The release of neurofilament proteins into biofluids can provide evidence that axons have been injured. Measurements should therefore be interpreted as indicators of axonal damage within the context of the research question, rather than as a direct description of all neuronal changes. This signal supports studies of traumatic damage and neurodegenerative disease involving axonal pathology.
The available approach depends on the information being sought. Immunohistochemistry can detect Neurofilament 200 in tissue, immunofluorescence can visualize its distribution within neuronal structures, and immunoblotting can assess its presence in a sample. Together, these methods support complementary analyses of neuronal identification, filament organization, and changes in neurofilament-associated signals.
In neuronal development studies, Neurofilament 200 detection helps examine the appearance and organization of neuronal structures. In disease or injury research, investigators can assess altered neurofilament organization or protein release as evidence relevant to axonal pathology. These applications extend across neurodegenerative disease, traumatic damage, and questions about how neuronal architecture changes over time.