Spinal Cord Imaging

Spinal cord imaging uses medical imaging techniques to visualize the spinal cord, its surrounding structures, and pathological changes, supporting assessment of nervous system anatomy and function. Magnetic resonance imaging (MRI) generates detailed images by aligning hydrogen nuclei in a magnetic field and measuring radiofrequency signals, while diffusion-based sequences map water movement along white-matter tracts. In neuroscience, these approaches help investigate spinal cord injury, tumors, inflammation, vascular abnormalities, and neurodegenerative disease. Imaging findings can guide diagnosis, monitor disease progression, evaluate treatment responses, and link structural changes with motor and sensory deficits.

Spinal Cord Imaging - Related Videos

Research

JoVE Journal - Neuroscience

Two-photon Imaging of Cellular Dynamics in the Mouse Spinal Cord

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Cited by 5 •

2015

A new ex vivo preparation for imaging the mouse spinal cord. This protocol allows for two-photon imaging of live cellular interactions throughout the spinal cord.

Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats

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Cited by 55 •

2012

We describe two tactile sensory testing methods for acute or chronic periods of spinal cord injury in rats. These validated procedures can detect the development and maintenance of allodynia-like sensations.

Spinal Cord Window Implantation in a Mouse for In Vivo Imaging

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2025

Source: Pietruczyk, E. A., et al. Laminectomy and Spinal Cord Window Implantation in the Mouse. J. Vis. Exp. (2019)This video demonstrates the implantation of a spinal cord window in an anesthetized mouse for in vivo imaging. The protocol stabilizes the exposed spinal cord using a borosilicate cover glass and a 3D-printed backplate. This method enables high-resolution two-photon microscopy to study blood-brain barrier dynamics and neurovascular interactions.

In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy

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Cited by 42 •

2012

A minimally invasive protocol to stabilize the mouse spinal column and perform repetitive in vivo spinal cord imaging using two-photon microscopy is described. This method combines a spinal stabilization device and an anesthetic regimen to minimize respiratory-induced movements and produce raw imaging data that require no alignment or other post-processing.

Research

JoVE Journal - Neuroscience
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Diffusion Imaging in the Rat Cervical Spinal Cord

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Cited by 10 •

2015

The goal of this protocol is to obtain high-quality diffusion weighted magnetic resonance imaging (DWI) of the rat spinal cord for noninvasive characterization of tissue microstructure. This protocol describes optimizations of the MRI sequence, radiofrequency coil, and analysis methods to enable DWI images free from artifacts.

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