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Two-photon Imaging of Cellular Dynamics in the Mouse Spinal Cord
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JoVE Journal Neuroscience
Two-photon Imaging of Cellular Dynamics in the Mouse Spinal Cord

Two-photon Imaging of Cellular Dynamics in the Mouse Spinal Cord

DOI:
10.3791/52580-v

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10:44 min

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February 22, 2015

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, , , , , ,
1Molecular Biology and Biochemistry,University of California, Irvine, 2Physiology and Biophysics,University of California, Irvine, 3Neurobiology and Behavior,University of California, Irvine, 4University of California San Francisco Diabetes Center,University of California, San Francisco, 5Pathology,University of Utah

Chapters

  • 00:05Title
  • 01:59Removal of Spinal Cord
  • 04:59Preparation of Spinal Cord for Imaging
  • 06:24Two-photon (2P) Imaging of Ventral Spinal Cord
  • 09:06Results: Representative 2P Image Acquired Inside the Ventral Spinal Cord
  • 10:02Conclusion

Summary

Automatic Translation

Automatic Translation

A new ex vivo preparation for imaging the mouse spinal cord. This protocol allows for two-photon imaging of live cellular interactions throughout the spinal cord.

Tags

Keywords: Two-photon ImagingCellular DynamicsMouse Spinal CordNeural Precursor CellsTransplantationDemyelinating DisordersOligodendrocytesRemyelinationIntravital ImagingAxonsFluorescent Proteins

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