Spiral Ganglion Neurons
Spiral Ganglion Neurons - Related Videos
02:35
Culturing Murine Spiral Ganglion Neuron Explants on Multielectrode Arrays
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2025
This video demonstrates the process for isolating spiral ganglion explants from the inner ear and co-culturing them with the organ of Corti on a multielectrode array. Spiral ganglia are carefully extracted and sectioned into smaller tissue sections or explants. These explants are cultured on the multielectrode array's active area to promote neuronal process outgrowth.
07:51
Spiral Ganglion Neuron Explant Culture and Electrophysiology on Multi Electrode Arrays
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Cited by 5 •
2016
We present a protocol to culture primary murine spiral ganglion neuron explants on multi electrode arrays to study neuronal response profiles and optimize stimulation parameters. Such studies aim to improve the neuron-electrode interface of cochlear implants to benefit hearing in patients as well as the energy consumption of the device.
02:40
Analyzing Neural Activity in Primary Murine Spiral Ganglion Neurons Using Multielectrode Arrays
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2025
This video demonstrates the recording of spontaneous and stimulation-dependent neural activity from primary murine spiral ganglion neuron (SGN) cultures on a multielectrode array (MEA). It involves washing and preparing the MEA, recording spontaneous neural activity, and applying controlled electrical stimuli to identify stimulation-dependent neuronal activity.
05:14
Differentiating Immortalized Multipotent Otic Progenitors into Spiral Ganglion Neurons and Evaluating the Differentiation
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2025
The video demonstrates the differentiation of immortalized multipotent otic progenitors (iMOPs) into spiral ganglion neurons (SGNs) and immunofluorescence-based confirmation of the differentiation. The iMOPs are plated onto culture substrate-coated coverslips in a neuronal differentiation medium for differentiation into SGNs. The differentiated cells are labeled with antibodies specific for neuronal differentiation markers and analyzed under a microscope to confirm differentiation.
04:41
Isolating Vestibular Ganglion Neurons From a Mouse Inner Ear
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2025
This video showcases a method for isolating vestibular neurons from a mouse pup's inner ear. It meticulously outlines the steps involved in dissecting the inner ear, harvesting the vestibular ganglion, and subsequently dissociating the ganglion into single cells. These cells are then cultured on glass-bottom dishes to establish a vestibular neuron culture.
FAQs
Sound-driven activity in cochlear hair cells promotes neurotransmitter release onto the peripheral processes of spiral ganglion neurons. This input changes neuronal firing, and the resulting signals travel through their central axons, which gather to form the auditory nerve. The sequence links sensory transduction in the cochlea with transmission of auditory information to the brain.
Their bipolar organization separates input and output functions within a single neuron. One process extends toward cochlear sensory hair cells and receives synaptic information, while the opposite process carries the resulting neural signal centrally toward the auditory nerve. This arrangement supports directional communication between the sensory epithelium and the brain.
Neurotransmitter release from activated hair cells provides the immediate synaptic signal that drives spiral ganglion neuron activity. The strength and timing of this input influence when these neurons fire, allowing cochlear events to be represented as neural signals. Examining this step helps researchers study how auditory information is encoded before reaching the brain.
Their condition and performance are central to understanding hearing impairment because damage or dysfunction can disrupt transmission between cochlear hair cells and the brain. Research examines neuronal survival and signaling alongside hair-cell activity to clarify mechanisms associated with sensorineural deafness. These studies can identify targets for preserving auditory pathways and improving therapeutic strategies.
Cochlear implants are studied partly through their effects on the neurons that connect cochlear sensory structures with the auditory nerve. Assessing those neural responses helps researchers understand how implant-generated stimulation engages the auditory pathway. This context supports efforts to improve hearing technologies and to determine how the condition of the neural population may influence outcomes.
Research targets include neuroprotection, which seeks to maintain neuronal survival and function, and potential regeneration, which aims to restore damaged neural elements. These directions are relevant to sensorineural deafness because effective auditory transmission depends on an intact pathway from cochlear sensory cells to the brain. Findings may guide future treatments and hearing technology development.