Transgenic Mice Retina
Transgenic Mice Retina - Related Videos
09:32
Bioluminescence Imaging of Neuroinflammation in Transgenic Mice After Peripheral Inoculation of Alpha-Synuclein Fibrils
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Cited by 1 •
2017
Peripheral injection of alpha-synuclein fibrils into the peritoneum or tongue of Tg(M83+/-:Gfap-luc+/-) mice, which express human alpha-synuclein with the familial A53T mutation and firefly luciferase, can induce neuropathology, including neuroinflammation, in their central nervous system.
04:32
Two-Photon In Vivo Imaging of the Mouse Retina
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2025
Source: Wang, Z., et al. Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina. J. Vis. Exp. (2021).This video demonstrates a technique for in vivo retinal imaging using two-photon microscopy. Anesthetized mice are prepared for imaging by securing their heads, Applying eye lubricant, and placing a coverslip over the eye. Retinal ganglion cells (RGCs) expressing fluorescent proteins are first visualized using epifluorescence for alignment. Finally, two-photon imaging is employed,...
05:53
In vivo Electroporation of Developing Mouse Retina
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Cited by 33 •
2011
A method for the incorporation of plasmid DNA into murine retinal cells for the purpose of performing either gain- or loss of function studies in vivo is presented. This method capitalizes on the transient increase in permeability of cell plasma membranes induced by the application of an external electrical field.
07:32
Assessment of Vascular Regeneration in the CNS Using the Mouse Retina
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Cited by 7 •
2014
The rodent retina has long been recognized as an accessible window to the brain. In this technical paper we provide a protocol that employs the mouse model of oxygen-induced retinopathy to study the mechanisms that lead to failure of vascular regeneration within the central nervous system after ischemic injury. The described system can also be harnessed to explore strategies to promote regrowth of functional blood vessels within the retina and CNS.
07:53
Split Retina as an Improved Flatmount Preparation for Studying Inner Nuclear Layer Neurons in Vertebrate Retina
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2024
This work presents an alternative flatmount retina preparation in which the removal of photoreceptor cell bodies enables faster antibody diffusion and improved patch pipette access to inner retinal neurons for immunohistochemistry, in situ hybridization, and electrophysiology experiments.
FAQs
Introducing the chosen construct into the early embryo allows the genetic alteration to become part of the mouse genome as the animal develops. Researchers can therefore relate that change to retinal structure, function, development, and neural signaling over time, rather than examining only a later, isolated effect.
Some models direct the genetic change specifically toward retinal cells, helping investigators connect gene activity with effects in the retina itself. This focus supports analysis of how altered genes influence retinal organization and function while also clarifying links between local retinal changes, visual pathways, and observable behavior.
These models connect a defined genetic alteration with changes in retinal cells, neural signaling, disease mechanisms, and visual function. By studying those relationships under controlled conditions, researchers can investigate how genes contribute to retinal development or inherited disorders and identify measurable consequences associated with particular genetic changes.
Researchers first introduce a selected genetic construct into the early embryo. After the modification becomes part of the mouse genome, they examine relevant retinal effects, including changes in cells, structure, function, or signaling. They can then relate those findings to visual pathways, behavior, development, or disease-related outcomes.
The models support assessment of retinal cells and structure, retinal function, neural signaling, visual pathways, and behavior. These outcomes provide several connected levels of evidence, allowing investigators to associate a genetic change with cellular effects as well as broader consequences for visual processing and observable responses.
They are useful when researchers need to examine how a specific genetic change contributes to inherited disorders or other retinal disease mechanisms. Controlled conditions allow the altered gene, retinal effects, neural consequences, and behavioral outcomes to be considered together, helping investigators trace disease-related relationships within the visual system.
A treatment can be evaluated against measurable effects associated with the model's genetic alteration. Researchers can examine whether relevant retinal cells, retinal function, visual pathways, or behavior show changes after the intervention. This approach links treatment assessment to a defined genetic context and to outcomes spanning cellular and visual-system levels.