Thyroxine Metabolism
Thyroxine Metabolism - Related Videos
07:35
Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli
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Cited by 47 •
2014
Elastin-like polypeptides are stimulus-responsive biopolymers with applications ranging from recombinant protein purification to drug delivery. This protocol describes the purification and characterization of elastin-like polypeptides and their peptide or protein fusions from Escherichia coli using their lower critical solution temperature phase transition behavior as a simple alternative to chromatography.
09:42
Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides
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Cited by 6 •
2012
We describe here a technique that is now routinely used to isolate stably bound ribosome nascent chain complexes (RNCs). This technique takes advantage of the discovery that a 17 amino acid long SecM "arrest sequence" can halt translation elongation in a prokaryotic (E. coli) system, when inserted into (or fused to the C-terminus) of virtually any protein.
10:15
Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
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Cited by 2 •
2012
A technique to identify translational pause sites on mRNA is described. This procedure is based on isolation of nascent polypeptides accumulating on ribosomes during in vitro translation of a target mRNA, followed by the size analysis of the nascent chains using a denaturing gel electrophoresis.
05:41
Metabolic Profile Analysis of Zebrafish Embryos
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Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
10:19
An Introduction to Cell Metabolism
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2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
FAQs
Deiodinases regulate the biological direction of T4 signaling by removing iodine atoms. This conversion can produce T3, the more active form, or reverse T3, an inactive form. Because these pathways alter how much active hormone is available in peripheral tissues, they help determine the strength and functional consequences of thyroid hormone signaling.
Negative feedback helps stabilize thyroid hormone production. The hypothalamus and pituitary adjust signals that influence thyroid secretion in response to circulating hormone availability. This regulatory relationship prevents the system from operating independently of its output and provides a mechanism for maintaining hormone levels relevant to energy use, development, and nervous-system function.
These stages determine when and where thyroid hormone signals are available. Production supplies T4, transport distributes it, tissue-level activation can generate T3, and clearance removes hormone or its metabolites. Considering the stages together is important because altered availability may influence nervous-system function even when the initial hormone is produced normally.
Changes in thyroid hormone availability may influence brain development and nervous-system activity, with possible effects on mood, attention, and cognition. These relationships give behavioral researchers a biological context for symptoms that may appear psychological in presentation. They also support investigations into how endocrine pathways relate to mental health and behavior.
Researchers can consider thyroid hormone availability as one biological factor when examining behavioral symptoms. Measures or evidence related to hormone production, activation, or clearance may help place changes in mood, attention, or cognition within an endocrine context. This approach supports interpretation of behavior without treating thyroid pathways as the sole explanation for mental or cognitive outcomes.
The pathway supports research on whether differences in thyroid hormone availability are associated with mood, attention, cognition, brain development, or broader mental-health outcomes. It is especially relevant when studies connect endocrine regulation with behavior, because the conversion of T4 into active or inactive forms provides a mechanism that may help explain observed psychological patterns.