Barkodlu Maya Kütüphaneler Rekabet Genomik Ekranlar
19.5K görüntüleme
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44 kez atıf
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11:59 dk
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11 Ağustos 2011
DOI :10.3791/2864-v
11 Ağustos 2011
•19.5K görüntüleme
Andrew M. Smith*1,2 , Tanja Durbic*2,3 , Julia Oh*4 , Malene Urbanus1,2 , Michael Proctor5 , Lawrence E. Heisler2,3 , Guri Giaever2,6 , Corey Nislow1,2,3
1Banting and Best Department of Medical Research and Department of Molecular Genetics, University of Toronto, 2Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 3Donnelly Sequencing Centre, University of Toronto, 4Genetics and Molecular Biology Branch, National Human Genome Research Institute, NIH, 5Stanford Genome Technology Center, Stanford School of Medicine, Stanford University , 6Department of Pharmaceutical Sciences, University of Toronto
* These authors contributed equally
Biz gen-ilaç ve gen-çevre etkileşimleri anlamak için kapsamlı, tarafsız genom ekranlar geliştirdik. Bu mutant koleksiyonlarını tarama yöntemleri sunulmaktadır.
Chapters in this video
0:05
Title
1:55
Combining Individual Mutants into a Single Pool
3:23
Experimental Pool Growth
4:56
Genomic DNA Extraction, PCR and Microarray Hybridization
8:33
Sequencing Barcoded Yeast Strains
9:22
Genomic Screening Results
11:03
Conclusion
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