Name: Akciğer Kanserinin Sinjeneik Ortotopik Fare Modelini Oluşturmak için Minimal İnvaziv Bir Yöntem (Video) | JoVE Journal
Uploaded: 2025-08-19T13:40:00.000+00:00
Duration: 7 min 46 s
Description: Öğrenin minimal invaziv ortotopik akciğer kanseri modeli oluşturmayı. Biyolüminesans görüntüleme ile erken tümör tespiti sağlayan bu yöntem, yeni kanser tedavilerinin preklinik geliştirilmesine olanak tanır.

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5 minutes

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akciğer kanserinin sinjeneik ortotopik fare modelini oluşturmak için minimal İnvaziv bir yöntem

A Minimally Invasive Method for Generating a Syngeneic Orthotopic Mouse Model of Lung Cancer

Akciğer Kanserinin Sinjeneik Ortotopik Fare Modelini Oluşturmak için Minimal İnvaziv Bir Yöntem

Non-small cell lung cancer (NSCLC) continues to be the number one cause of cancer-related death for both women and men worldwide. More information needs ...

a-minimally-invasive-method-for-generating-syngeneic-orthotopic-mouse

Research

JoVE Journal

Cancer Research

Kanser Araştırmaları

Video, bir fare modelinde meme kanseri ortotopik aşılama tekniğini göstermektedir. Bu teknik, deri altı veya cerrahi olmayan enjeksiyona kıyasla tümör boyutu ve şeklinde daha az değişkenlik ile yüksek bir tümör oluşumu oranı üretir.

Ortotopik Enjeksiyon: Dokuya Özgü Kanser Hücrelerinin Yetişkin Bir Fareye İmplante Edilmesi

Birincil invaziv kadavradan mesane kanseri zenograftlarını aşılamak için kurulan teknik laparotomi ve mesane seferberlik gerektirir. Bu prosedür, fareler üzerinde önemli morbidite uğratan teknik açıdan zor ve zaman alıcıdır. Bu nedenle perkütan yaklaşım ultrason rehberlik kullanarak, yüksek hassasiyet geliştirdi.

Kemirgen Ortotopik Mesane ksenogratflarının Minimal İnvaziv kurulması

Kanserin metastaz kansere bağlı ölümlerin önemli bir nedenidir. Biz solid organ tümörlerinin metastaz mekanizmaları çalışmak için bir "hasta" gibi ortotopik singenik fare modeli sistemi kurulması için hayatta kalma cerrahi yönteminin derinlemesine bir açıklama sağlar.

Bir &quot;Hasta gibi&quot; Ortotopik singenik Fare Modeli Hepatoselüler karsinomu metastazı

Mikro bilgisayarlı tomografi kullanarak bir adenokarsinom fare modelinin akciğerlerinde tümör nodülü gelişiminin saptanması için bir yöntem ve bunun zaman içinde ve tedaviye yanıt olarak nodül boyutundaki değişiklikleri izlemek için kullanımını açıklıyoruz. Değerlendirmenin doğruluğu son nokta histolojik kantizasyonu ile doğrulandı.

Tümör Gelişim Değerlendirme Mikro bilgisayarlı Tomografi ile ve Takibi Tedavi Yanıtın Akciğer Kanseri Fare Modeli

Metastazı, kanserin virülansında derin bir rol oynar ve ölümlerin tahminen %90'ını oluşturur.  Farelerde metastatik melanom modeli için terapötik ajanların bu klinik fenomene karşı etkinliğini belirlemek için yararlı olan bir protokol sunuyoruz.

Singenik Fare Melanom Hücreleri Enjeksiyon Akciğerler Onların Metastatik Potansiyeli belirleme

Biz önceden airways yaralanma ile Klima tarafından fare ciğerlerine akciğer kanser hücrelerinin orthotopic engraftment önemli ölçüde geliştirmek için bir yöntem açıklanmaktadır. Bu yaklaşım aynı zamanda akciğer microenvironment, metastatik yayılması, akciğer kanseri komorbiditeler içinde stromal etkileşimleri çalışmaya uygulanabilir ve daha verimli bir şekilde hasta oluşturmak için xenografts türetilmiş.

Bleomycin ile fareler Airways önceden Merkezi Klima Orthotopic akciğer kanseri hücre Engraftment verimliliğini artırır

Biz bir fare orthotopic meme kanseri modeli ve radikal mastektomi modeli insan meme kanseri ilerleme taklit etmek için tümör yükünü ölçmek için bioluminescence teknolojisi ile tanıtmak.

Fare Orthotopic metastatik meme kanseri Model oluşturma ve fare radikal mastektomi gerçekleştirme

Burada küçük hücreli dışı akciğer kanseri çalışma için zaman ve maliyet azaltma mankenlik minimal invaziv syngeneic orthotopic nakli manken fare akciğer adenokarsinom hücre açıklayın.

Orthotopic nakli PD-L1 ifade çalışmaya Syngeneic akciğer adenokarsinom hücre

Bu protokol, ortopik osteosarkom ve pulmoner metastaz lezyonlarını taşıyan fare modelleri oluşturmak için intratibia osteosarkom hücre enjeksiyonunu tanımlamaktadır.

Ortotopik Osteosarkom ve Akciğer Metastazı Fare Modelleri Oluşturmak için İntratibiyal Osteosarkom Hücre Enjeksiyonu

Bu çalışma, floresan A549-iRFP hücrelerinin çok hücreli sferoidlerinin intrapulmoner inokülasyonuna dayanan ortotopik küçük hücreli dışı akciğer kanseri (KHDAK) modelini sunmaktadır. Model, klinik NSCLC aşamalarını özetler ve uzun dalga boylu floresanın dinamik in vivo izlemesine göre sisplatine yanıt verir.

Küçük Hücreli Dışı Akciğer Kanserinde Hastalık Progresyonunu Taklit Eden ve İlaç Aktivitelerini Tahmin Eden Ortotopik Ksenogreft Modelinin Oluşturulması

Öğrenin minimal invaziv ortotopik akciğer kanseri modeli oluşturmayı. Biyolüminesans görüntüleme ile erken tümör tespiti sağlayan bu yöntem, yeni kanser tedavilerinin preklinik geliştirilmesine olanak tanır.

Akciğer Kanserinin Sinjeneik Ortotopik Fare Modelini Oluşturmak için Minimal İnvaziv Bir Yöntem

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