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The Preparation of Drosophila Embryos for Live-Imaging Using the Hanging Drop Protocol
 

The Preparation of Drosophila Embryos for Live-Imaging Using the Hanging Drop Protocol

Article doi: 10.3791/1206
March 13th, 2009

Summary March 13th, 2009

A simple, inexpensive, and effective method of preparing Drosophila embryos for live-imaging analysis is presented. Our protocol provides humidity and gas exchange and does not compress the Drosophila embryo. This method is suitable for GFP-based live imaging of Drosophila embryos using a stereomicroscope or upright compound microscope.

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