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TIRF显微镜视网膜双极细胞的影像胞外分泌
JoVE Journal
Biology
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JoVE Journal Biology
Imaging Exocytosis in Retinal Bipolar Cells with TIRF Microscopy
DOI:

11:54 min

June 09, 2009

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Chapters

  • 00:05Title
  • 01:00Introduction
  • 01:29Dissection and Bipolar Cell Isolation
  • 05:11Bipolar Cell Loading and Wash Out
  • 08:26Patch Clamping and TIRFM Imaging
  • 10:38Representative TIRFM Imaging Results
  • 11:23Conclusion

Summary

Automatic Translation

在这个视频中,我们演示了如何标记和可视化单突触囊泡的胞吐贩卖和使用总内部反射荧光(TIRF)显微镜在金鱼视网膜双极细胞。

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