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TIRF显微镜视网膜双极细胞的影像胞外分泌

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Biology

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JoVE Journal Biology

Imaging Exocytosis in Retinal Bipolar Cells with TIRF Microscopy

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TIRF显微镜视网膜双极细胞的影像胞外分泌

DOI:

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11:54 min

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June 09, 2009

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Christina Joselevitch, David Zenisek

¹Cellular and Molecular Physiology,Yale University School of Medicine

Chapters

00:05Title
01:00Introduction
01:29Dissection and Bipolar Cell Isolation
05:11Bipolar Cell Loading and Wash Out
08:26Patch Clamping and TIRFM Imaging
10:38Representative TIRFM Imaging Results
11:23Conclusion

Summary

Automatic Translation

在这个视频中，我们演示了如何标记和可视化单突触囊泡的胞吐贩卖和使用总内部反射荧光（TIRF）显微镜在金鱼视网膜双极细胞。

Tags

TIRF Microscopy Exocytosis Retinal Bipolar Cells Synaptic Vesicles Goldfish Retina Patch Clamping Pre-synaptic Voltage Synaptic Release Fluorescent Dye FM 1-43®Depolarizes Endocytosis Glutamatergic Vesicles Patch Pipette Solution Rhodamine-based Peptide Synaptic Ribbon Protein RIBEYE Active Zones

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