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Primäre Kultur und Plasmid Elektroporation der Murine Corti-Organ.
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Primäre Kultur und Plasmid Elektroporation der Murine Corti-Organ.

Article DOI: 10.3791/1685
February 4th, 2010 •
1,2,3, 1,2, 1,2,4
1Department of Otology and Laryngology, Harvard Medical School, 2Eaton-Peabody Laboratory, Massachusetts Eye and Ear Infirmary, 3Department of Communication Sciences and Disorders, Emerson College, 4Program in Speech and Hearing Bioscience and Technology, Division of Health Science and Technology, Harvard

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February 4th, 2010

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Dieses Verfahren beschreibt eine Methode zur Isolierung und Kultivierung der murinen Corti-Organ mit oder ohne die Spirale Limbus und Spiralganglions Neuronen. Wir zeigen auch ein Verfahren zur Expression eines exogenen Reporter-Gen in das Corti-Organ Explantat durch Elektroporation.

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