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Journal
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Biology
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的RNA分离方法的改进火炬松(
P。火炬松
L)和其他针叶树种
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Biology
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JoVE Journal
Biology
An Improved Method of RNA Isolation from Loblolly Pine (
P. taeda
L.) and Other Conifer Species
Please note that all translations are automatically generated.
Click here for the English version.
的RNA分离方法的改进火炬松(
P。火炬松
L)和其他针叶树种
DOI:
10.3791/1751-v
•
08:25 min
•
February 22, 2010
•
W. Walter Lorenz
,
Yuan-Sheng Yu
,
Jeffrey F. D. Dean
1
Warnell School of Forestry and Natural Resources
,
University of Georgia (UGA)
Chapters
00:00
Title
00:23
Tissue Harvest
00:45
Collection of Phloem
01:07
Collection of Secondary Xylem
01:26
Collection of Reaction Wood: Compression and Opposite Secondary Xylem
02:21
Liquid Nitrogen Storage
02:46
RNA Isolation: Tissue Preparation
03:20
RNA Isolation Day 1
04:41
RNA Isolation Day 2
06:35
RNA Precipitation
07:16
Final Resuspension and Quantification
08:02
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Summary
Automatic Translation
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Automatic Translation
许多植物组织中,包括从火炬松的韧皮部及木质部(<em>火炬松</em> L),含有酚和干扰RNA纯化的多糖高的水平。此演示文稿讨论的收获种植现场组织和隔离的芯片和其他基因组分析的RNA质量足够技术。
Tags
RNA Isolation
Conifer Species
Loblolly Pine
Pinaceae Family
Phenolic Compounds
Polysaccharides
RNA Purification
Organic Extraction Steps
Microarray Analysis
Genomic Analysis
Field-collected Samples
RNA Degradation
Total RNA Yields
Oxidizing Agents
Contaminants
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