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Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells
 

Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells

Article DOI: 10.3791/1899
April 27th, 2010

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Summary April 27th, 2010

We describe a method for analysis of the alteration of N-linked glycans through the early life of glycoproteins after their biosynthesis in mammalian cells. This is achieved by pulse-chase analysis of metabolically labeled glycans, enzymatic release from glycoproteins and examination by HPLC.

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