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SDS-PAGE/Immunoblot الكشف عن Multimers Aβ في الأنسجة البشرية القشرية الخليط باستخدام مستضد استرجاع حاتمة
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Chapters
- 00:00Title
- 00:47Introduction
- 01:20Preparation of Clarified Tissue Homogenates
- 02:43SDS-PAGE Sample Preparation and Electrophoresis
- 04:12Transferring Proteins from Gels to Membranes
- 05:35Antigen-Epitope Retrieval and Immunoblotting
- 08:13Aβ Multimers by SDS-Page Analysis Using the 6E10 Antibody
- 09:53Conclusion
Tags
SDS-PAGE/ImmunoblotAβ MultimersAntigen-epitope RetrievalAnomalous FoldingPolymerizationβ-amyloid PeptideNeurodegenerative CascadeAlzheimer’s Disease PathogenesisSelf-aggregationAmyloid FibrilsCerebral Senile PlaquesLow Molecular WeightSoluble Aβ MultimersToxic MultimersProteopathiesSDS-polyacrylamide Gel ElectrophoresisImmunoblottingAβ-specific AntibodiesHighly Concentrated Cortical HomogenatesSmall Pore-size Nitrocellulose MembranesClarified Human Cortical Homogenates
