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Neuroscience

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JoVE Journal Neuroscience
Rilevazione SDS-PAGE/Immunoblot di multimeri Aβ in Human tessuto corticale omogenati utilizzando Antigen-epitopi recupero
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Rilevazione SDS-PAGE/Immunoblot di multimeri Aβ in Human tessuto corticale omogenati utilizzando Antigen-epitopi recupero

Article DOI: 10.3791/1916 • 10:49 min
April 23rd, 2010 •
1, 2, 3, 1,4
1Yerkes National Primate Research Center, Emory University, 2Department of Neurology, Institute of Clinical Medicine, Tsukuba University, 3Department of Pathology, New York University School of Medicine, 4Department of Neurology, Emory University

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April 23rd, 2010

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Abbiamo descritto una tecnica per la preparazione di omogenati chiarito umano corticale, la separazione delle proteine ​​mediante SDS-PAGE, recupero antigene e immunoblotting con un anticorpo per il peptide Aβ. Usando questo protocollo, abbiamo costantemente rilevare Aβ monomerici e multimerica nel tessuto corticale da esseri umani con patologia di Alzheimer.

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