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T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis
JoVE Journal
Biology
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JoVE Journal Biology
T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis

T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis

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16:40 min

July 31, 2010

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16:40 min
July 31, 2010

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Summary

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Ion mobility-mass spectrometry is an emerging gas-phase technology that separates ions, based on their collision cross-section and mass. The method provides three-dimensional information on the overall topology and shape of protein complexes. Here, we outline a basic procedure for instrument setting and optimization, calibration of drift times, and data interpretation.

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