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JoVE Journal
Biology

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T-wave Ion Mobility-mass Spectrometry
 

T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis

Article DOI: 10.3791/1985
July 31st, 2010

Chapters

Summary July 31st, 2010

Ion mobility-mass spectrometry is an emerging gas-phase technology that separates ions, based on their collision cross-section and mass. The method provides three-dimensional information on the overall topology and shape of protein complexes. Here, we outline a basic procedure for instrument setting and optimization, calibration of drift times, and data interpretation.

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