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Using Touch-evoked Response and Locomotion Assays to Assess Muscle Performance and Function in Zebrafish
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Using Touch-evoked Response and Locomotion Assays to Assess Muscle Performance and Function in Zebrafish

Using Touch-evoked Response and Locomotion Assays to Assess Muscle Performance and Function in Zebrafish

DOI:
10.3791/54431-v

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09:40 min

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October 31, 2016

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, , ,
1School of Biological Sciences,Monash University

Chapters

  • 00:05Title
  • 00:43Touch-evoked Response Assay
  • 01:53Quantification of Swimming Behavior
  • 03:3210 Min Swim Test Locomotion Assay
  • 07:01Results: Muscle Performance and Locomotion in Zebrafish Embryos
  • 08:47Conclusion

Summary

Automatic Translation

Automatic Translation

Zebrafish are an excellent model to study muscle function and disease. During early embryogenesis zebrafish begin regular muscle contractions producing rhythmic swimming behavior, which is altered when the muscle is disrupted. Here we describe a touch-evoked response and locomotion assay to examine swimming performance as a measure of muscle function.

Tags

Keywords: ZebrafishMuscle PerformanceTouch-evoked ResponseLocomotion AssayNeuromuscular ResearchHigh-throughputVideo RecordingMechanosensory StimulusSwimming BehaviorMaximum Acceleration

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