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Ultrasound-Guided Microinjection into the Mouse Forebrain In Utero at E9.5
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Ultrasound-Guided Microinjection into the Mouse Forebrain In Utero at E9.5

Ultrasound-Guided Microinjection into the Mouse Forebrain In Utero at E9.5

DOI:
10.3791/2047-v

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07:47 min

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November 13, 2010

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,
1Institute for Cell Engineering Neuroregeneration and Stem Cell Programs,Johns Hopkins University School of Medicine, 2Departments of Neurology, Neuroscience, and Oncology,Johns Hopkins University School of Medicine

Chapters

  • 00:00Title
  • 01:56Exposure of the Embryos
  • 03:45Loading Virus into a Microinjection Needle
  • 04:49Ultrasound-guided Viral Injection
  • 06:25Representative Results
  • 07:05Conclusion

Summary

Automatic Translation

Automatic Translation

In utero survival surgery in mice permits the molecular manipulation of gene expression during development. Here we describe the use of high-frequency ultrasound imaging to guide the injection of retroviral vectors into the mouse brain at embryonic day (E) 9.5.

Tags

Ultrasound-guidedMicroinjectionMouse ForebrainIn UteroE9.5Survival SurgeryGene ExpressionEmbryogenesisMicroinjection NeedleRetroviral VectorsVentricular SystemLaparotomySaline BathInjected EmbryosAmniotic FluidNeural TubeBrain RegionsViral InfectionCell Transplantation

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