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Induction and Assessment of Class Switch Recombination in Purified Murine B Cells
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Induction and Assessment of Class Switch Recombination in Purified Murine B Cells

Induction and Assessment of Class Switch Recombination in Purified Murine B Cells

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09:49 min

August 13, 2010

DOI:

09:49 min
August 13, 2010

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Summary

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Following antigen exposure, subpopulations of activated B cells undergo a process known as class switch recombination (CSR) to produce antibody isotypes with distinct effector functions. The protocol outlined in this report explains how CSR can be induced and analyzed in vitro for the purposes of studying B cell function.

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