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Visualization of Mitochondrial DNA Replication in Individual Cells by EdU Signal Amplification
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Chapters
- 00:00Title
- 00:58Introduction
- 01:56Preparation of Neurons
- 03:38Click-iT 5-ethynyl-2-deoxyuridine (EdU) Labeling
- 05:31Tyramide Signal Amplification (TSA) of EdU Signal
- 07:09Visualization of Mitochondrial DNA Replication as a Marker for Mitochondiral Biogenesis
- 08:37Conclusion
We developed a sensitive technique to label newly synthesized mitochondrial DNA (mtDNA) in individual cells in order to study mtDNA biogenesis. The technique combines the incorporation of EdU together with a tyramide signal amplification (TSA) protocol to visualize mtDNA replication within subcellular compartments of neurons.
Tags
VisualizationMitochondrial DNA ReplicationCellsEdU Signal AmplificationMitochondriaCellular EnergyMitochondrial BiogenesisMtDNA Replication RateSensitive TechniqueLabelingNewly Synthesized MtDNAIndividual CellsMtDNA Biogenesis Study5-ethynyl-2′-deoxyuridine (EdU)Tyramide Signal Amplification (TSA)Visualization Of MtDNA ReplicationSubcellular CompartmentsNeuronsThymidine Analogs5-bromo-2-deoxyuridine (BrdU)Click ReactionAcid TreatmentsEnzyme DigestsEdU Incorporation ComparisonCellular MarkersMechanisms Of Mitochondrial Biogenesis RegulationPathogenesis Associated With Drug ToxicityAgingCancerNeurodegenerative Diseases
