Neuroscience
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Visualization of Mitochondrial DNA Replication in Individual Cells by EdU Signal Amplification
Chapters
Summary November 15th, 2010
We developed a sensitive technique to label newly synthesized mitochondrial DNA (mtDNA) in individual cells in order to study mtDNA biogenesis. The technique combines the incorporation of EdU together with a tyramide signal amplification (TSA) protocol to visualize mtDNA replication within subcellular compartments of neurons.
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