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Chapters
- 00:05Title
- 01:46Reagent Set-up and Preparation of Fly Cages
- 02:52Collections of Synchronous Embryos
- 03:36Collecting, Washing, Homogenizing Embryos, and Plating of Cells
- 06:35Primary-Cell RNAi for Cells Grown in a 384-well Plate
- 07:48Representative Images of Cultured Cells
- 10:30Conclusion
We provide a detailed protocol for preparing primary cells dissociated from Drosophila embryos. The ability to carry out the effective RNAi perturbation, together with other molecular, biochemical and cell imaging methods will allow a variety of questions to be addressed in Drosophila primary cells.
